Protein Info for A4249_RS12275 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: cobaltochelatase subunit CobT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 627 PF06213: CobT" amino acids 6 to 268 (263 residues), 265.6 bits, see alignment E=5.4e-83 TIGR01651: cobaltochelatase, CobT subunit" amino acids 9 to 612 (604 residues), 872.7 bits, see alignment E=8e-267 PF11775: CobT_C" amino acids 393 to 610 (218 residues), 350.3 bits, see alignment E=4.5e-109

Best Hits

Swiss-Prot: 55% identical to COBT_SINSX: Aerobic cobaltochelatase subunit CobT (cobT) from Sinorhizobium sp.

KEGG orthology group: K09883, cobaltochelatase CobT [EC: 6.6.1.2] (inferred from 76% identity to bsb:Bresu_0711)

MetaCyc: 55% identical to CobT (Pseudomonas denitrificans (nom. rej.))

Predicted SEED Role

"Aerobic cobaltochelatase CobT subunit (EC 6.6.1.2)" in subsystem Coenzyme B12 biosynthesis or Conenzyme B12 related Hypothetical: Clusters with cobST (EC 6.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.6.1.2

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HZ53 at UniProt or InterPro

Protein Sequence (627 amino acids)

>A4249_RS12275 cobaltochelatase subunit CobT (Brevundimonas sp. GW460-12-10-14-LB2)
MAAPDTPAETFKRALGHAARALAEQPELEVVFGSDGPKLSNGVLTLPHPPRDPSGPESAS
LRGQADRLALRLANHDAAVNSRMRPADAKAAEVFDAVEQARVEAFGSEHLAGVRDNLGAA
LITRLEKTGALRISDADRVPVSEAVALLVRERLTGRPAPDGARAMLDLVRANIEAKAGAK
LDALAGTAGDQEGFALKMREVLRALDLDPGDGRGEDTSEDPGDEEPDPQDPAADDDQDEQ
EEQDGGEGSQSMEGDADDQSSEQERESRPEGAPADPDGEGADDGPELQEGQQPNRQQTAD
DGRAVDFYRVFTTAFDEVVDAADLCDPVELDRLRALLDGQLAILSSVVSRLANKLQRRLL
AQQNRSWVFDLEEGMLDTARLTRIVTDPTAPLSFKAESESAFRDTVVTLLLDNSGSMRGR
PIMVAAVCADILARTLERCGVKVEILGFTTRAWKGGQSREAWITAGKPQNPGRLNDLRHI
IYKAADAPWRRAKKNLGLMMREGLLKENIDGEALQWAHGRLLARTEQRRILMVISDGSPV
DDSTQSANAALYLDKHLRQVIAEIEDRSPVELLAIGIGHDVTRWYRKALTIVDVEQLAGA
MTEKLAELFENEGAAGPTRRAKRRLAA