Protein Info for A4249_RS11470 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: ATP-dependent DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 951 transmembrane" amino acids 776 to 791 (16 residues), see Phobius details PF13307: Helicase_C_2" amino acids 776 to 914 (139 residues), 68.8 bits, see alignment E=3.2e-23

Best Hits

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 84% identity to bsb:Bresu_3313)

Predicted SEED Role

"DinG family ATP-dependent helicase YoaA" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160I1E1 at UniProt or InterPro

Protein Sequence (951 amino acids)

>A4249_RS11470 ATP-dependent DNA helicase (Brevundimonas sp. GW460-12-10-14-LB2)
MSDESVLSETRLTGAQPWANLPPALATPPGAGAVCDEAGARKIGRGAAEGFFSTGPVMVA
HASLTARRLGLTPPPRSTDLMDVLELYAFVRPARFCAPSPTGLALSLGSAEPKSAEEQAA
ALREAAGLLLRELTDPAYPEREAAYVLALTMQRAGWAWGERVVQALEAGGVRARQHRGSG
MDVWSRLTEWEDEAPRGEAGSTPVDSESARIRLEKLLQASGLDETRPTQSDYAAEAAFAF
SPRNEEGRPRMLLAEAGTGTGKTLGYLAPASLWAERNQGAAWISTYTRALQRQIDRESHS
LWPDPAERKKKAVIRKGRENYLCVLNLQDMVQAAQLGNGDLIGLALAGRWALHTRDGDMT
GGDFPGWLPGLFAMPAAHAAGAANLVDRRGECVHAACPHYRTCFVEKTIRASRRADLVIA
NHALVLTQAAFDGARSARGQKQDGETAALKRIVFDEGHHLFDAADSAFSACLSGQEAAEL
RRWIRGPEGRGRRGRGLEQRLGDMVNDNEAATKALQDAIRASAALPGEGVSGRVAPASGE
VNPIGPIEAFLAAALDQLRARTAETNTYGGPEFGMECALKPVTDPVAETARAAAQALAAI
EAPLLALSRHLEDILDDEAAELDGSQRSRIEGALRGLDRRARMTLPGWRSMLAALEDGGD
QPDPDFVDWLSAETAFGRIHDVALRRHWIDPTVPLEAAVIVPSHGVLVTSATLSDPLQED
PFDLARMRTGSARLIEPPRTLKVESPFDYAANSRVIVVNDLVKDDPRQTAAAMRELFLAA
GGGGLGLFTAIRRLKAVYERLGPDLAKSGVPLYAQHVDPLEVGALVDMFRVEENSCLLGT
DAVRDGVDVPGRSLRVLAFERVPWPRPDLLHKARRERLGGKGYDDALARARIAQAFGRLI
RRGDDKGVFLMLDAATPTRLFASLPPGTEVQRMGLADAVELTRSFLNPETT