Protein Info for A4249_RS11350 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 71 to 204 (134 residues), 62.9 bits, E=2e-21
• TIGR00093: pseudouridine synthase
  • amino acids 75 to 233 (159 residues), 115.3 bits, E=1.2e-37

Best Hits

KEGG orthology group: K06183, ribosomal small subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 81% identity to bsb:Bresu_3225)

Predicted SEED Role

"Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

See A0A160HYJ6 at UniProt or InterPro

Protein Sequence (244 amino acids)

>A4249_RS11350 pseudouridine synthase (Brevundimonas sp. GW460-12-10-14-LB2)
MKKTPTSRVDRLLGSMGYGSRAEMARMAKAGGIVLDGADLTDVSKRIAVTPDLPTRMEID
GEPLDPVQGLVILLNKPLGMTCSHKEEGALVYDILPRRWRARDPAISTIGRLDKQTTGLL
LLTDDGDLLHRVISPKRHVAKVYRAGLARPLTGTEGALFASGELVLEGEDKPLAPAVLEV
VTPTEALLTVTEGRYHQVRRMFAAAGNHVETLHRERLGGLALPADLAPGQWRLATADEVA
SIFA