Protein Info for A4249_RS10415 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: Na+/H+ antiporter NhaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 63 to 83 (21 residues), see Phobius details amino acids 97 to 120 (24 residues), see Phobius details amino acids 127 to 146 (20 residues), see Phobius details amino acids 155 to 176 (22 residues), see Phobius details amino acids 182 to 201 (20 residues), see Phobius details amino acids 210 to 238 (29 residues), see Phobius details amino acids 260 to 281 (22 residues), see Phobius details amino acids 289 to 314 (26 residues), see Phobius details amino acids 331 to 354 (24 residues), see Phobius details amino acids 365 to 387 (23 residues), see Phobius details PF06965: Na_H_antiport_1" amino acids 8 to 384 (377 residues), 395.1 bits, see alignment E=1.5e-122 TIGR00773: Na+/H+ antiporter NhaA" amino acids 9 to 382 (374 residues), 328 bits, see alignment E=3.8e-102

Best Hits

Swiss-Prot: 47% identical to NHAA_PSEE4: Na(+)/H(+) antiporter NhaA (nhaA) from Pseudomonas entomophila (strain L48)

KEGG orthology group: K03313, Na+:H+ antiporter, NhaA family (inferred from 71% identity to bsb:Bresu_0837)

Predicted SEED Role

"Na+/H+ antiporter NhaA type" in subsystem Na(+) H(+) antiporter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160I2K6 at UniProt or InterPro

Protein Sequence (414 amino acids)

>A4249_RS10415 Na+/H+ antiporter NhaA (Brevundimonas sp. GW460-12-10-14-LB2)
MARKLTLDFLKTEAASGSALALAAIAAVAIANSPWSADYFAWLKSYHVLQIGPIRLEETI
SDWIKEGLMAIFFLVVGLEIKYEIVRGELSDPKKLATPVLAALGGMAGPAAVYLLLSGAI
GAPHAGWPIPLATDIAFALAIFGFVGKGLPSSLRVFLLTLAIVDDLGAIALIAVLFSEGA
NWTPLFWALGALAVGAVVAHRIRIPAPFWVLGFAMVWYLTVLSGLSTSLTAVAFAMIVPI
KGRAEDGQSPLKEAMHDLHPWNAFLVLPLFAFAKAGVSFAGLSMEQAFAPLVIAIALGLF
FGKQIGVLGAAWLASALRIGARPTGASWLQVYGVSLLCGVGFTMSLFIGVLAFPGAVDSP
EQIEVKLGVIGGSILSAIAAALVLGFAGRARQVDPAALHSEVEPLGPKNGQKLT