Protein Info for A4249_RS10400 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: efflux RND transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1067 transmembrane" amino acids 15 to 34 (20 residues), see Phobius details amino acids 332 to 353 (22 residues), see Phobius details amino acids 360 to 377 (18 residues), see Phobius details amino acids 383 to 405 (23 residues), see Phobius details amino acids 431 to 451 (21 residues), see Phobius details amino acids 463 to 489 (27 residues), see Phobius details amino acids 545 to 564 (20 residues), see Phobius details amino acids 878 to 900 (23 residues), see Phobius details amino acids 907 to 927 (21 residues), see Phobius details amino acids 934 to 958 (25 residues), see Phobius details amino acids 981 to 1000 (20 residues), see Phobius details amino acids 1012 to 1036 (25 residues), see Phobius details PF00873: ACR_tran" amino acids 7 to 1036 (1030 residues), 767.8 bits, see alignment E=2.5e-234 PF03176: MMPL" amino acids 295 to 503 (209 residues), 31.4 bits, see alignment E=1.5e-11 PF02355: SecD_SecF" amino acids 314 to 482 (169 residues), 22.2 bits, see alignment E=1.3e-08

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 77% identity to bsb:Bresu_0839)

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160I0E2 at UniProt or InterPro

Protein Sequence (1067 amino acids)

>A4249_RS10400 efflux RND transporter permease subunit (Brevundimonas sp. GW460-12-10-14-LB2)
MGFQNISSWSIRNPIPIVLLFVVLTIAGTMSYFTLRTNNFPDVDLPVVAVTVVQAGAAPT
EMETQVTRLVEDAVAGLGSVKHITSVVNEGVSTTSIEFQLGVNLEKVTNDVRNAVSGIRQ
NLPADVQEPIVQRIEFTAIPFANYVVRAPGMSPEELSWFVDNTVAKRLLSVRGVSQISRD
GGVSREIRIKLDPARLAASGVTAAQVSNQLRASNINLPGGRGEIAGEEQAIRTVGSAKSV
EQLRETLIPIGSRSVRLGDLGQITDEWSEPRGRARFNGQEVVGFGVSRAIGSSEVDVYDR
VKAEIEKLDQERGDVAIEEVANTTEDVVNNFHASVETLVLGALLAIAVVFIFLRDWRATL
IAAVAMPLSLIPTFWVMDLTNQSLNVVTLLALSLTIGILVDDAIVEIENIVRHIRDGKAP
YPAAIEAADEIGLAVMATTATLIAVFAPTGFMPGVVGQFFKSFAIATCVSVLFSLLVART
LTPLMGAYLLKRDQGKEHKEPFWMGPYLKALNWALGDDWRKRRADHHPRASWFRRKVADR
MFDHRLWVLGMGILFFFLSIFMATKLPGEFIPVEDISRSTVTVQLPPGATLAETDSAVQR
INRELMKRPEVASVYSSVGSATTSFGPGGGGSAGEVRSANLTVNLVGRWDRKLNQQEFER
DMGPVLRQIPGARIQFGASGGGGGSSLLTIALVGDEANVLEPAAAKVEREMRSIPGLSNV
VSSASLVRPEILVTPRADVAALQGVSTQDISQVARVATLGDADQLLPKFNLGDRQVPIRV
MLTEQARSDLGVLENLQVPTASGATVPLSAVADITFGAGPNQINRLDRLRVANITAELTG
LTLSQGTQAVNALPAIKSLPQGVSQKPSGDAESFQELGMGFAFAIVTGILLMYVVLVLLF
KSFFHPITILAALPVSFGGAFFLLLVTGKSLSMPALIGIIMLTGIAAKNSILLVDYAIMA
MEKGMGRHDALMDAAHKRARPIIMTTMAMGLGMLPIAAAFGEGTAFRSPMAIAVIGGLIT
STALSLLFVPVVFSLIDGVKRRLEGRLNKMFHGQHGHEEPAAEDRPA