Protein Info for A4249_RS10010 in Brevundimonas sp. GW460-12-10-14-LB2
Annotation: 2-isopropylmalate synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 70% identity to bsb:Bresu_3089)Predicted SEED Role
"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- L-leucine biosynthesis (6/6 steps found)
- 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Pyruvate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.3.3.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160HY14 at UniProt or InterPro
Protein Sequence (416 amino acids)
>A4249_RS10010 2-isopropylmalate synthase (Brevundimonas sp. GW460-12-10-14-LB2) MITPESVRVSGVSRTEEIAADPNRVIVFDTTMRDGEQAPGFSMSAQAKVKMAQVLRDLGV DVIEAGFAAASPGDEDCIRRVAGEVEGPVFCSLSRANEKDIDATFRALAPAPKSHRRCHT FIGTSPIHRSAKLKMSTNEVLSTAVRSVEYARSLFDDVEFSAEDAFRTEPEFLADVLEAA ADAGARTLNVPDTVGYATPQEVRERFAALAARIKRRHPHVIFSAHCHDDLGMAVANSLAA VEGGARQIEGAINGIGERAGNCSIEEVIMALKVREDRYGVTTGADSRHLVRASKILCEIT ETVIARNKSVVGINAFAHEAGIHQHGMLADSRTYEIMRPQDVGFEGSWFVLGKHSGRHAI AKRAETIGRPIEGERLAAVVAGFKSRADQIGEINDAELIAIIDRVDGVSEVVAQYA