Protein Info for A4249_RS09675 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: siroheme synthase CysG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 PF13241: NAD_binding_7" amino acids 9 to 114 (106 residues), 57.2 bits, see alignment E=3.4e-19 TIGR01470: siroheme synthase, N-terminal domain" amino acids 10 to 204 (195 residues), 146.2 bits, see alignment E=1.1e-46 PF10414: CysG_dimeriser" amino acids 150 to 206 (57 residues), 58.3 bits, see alignment 7.7e-20 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 214 to 448 (235 residues), 287.5 bits, see alignment E=9.5e-90 PF00590: TP_methylase" amino acids 216 to 423 (208 residues), 189.1 bits, see alignment E=1.5e-59

Best Hits

Swiss-Prot: 50% identical to CYSG_METCA: Siroheme synthase (cysG) from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 77% identity to bsb:Bresu_1495)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HXW9 at UniProt or InterPro

Protein Sequence (460 amino acids)

>A4249_RS09675 siroheme synthase CysG (Brevundimonas sp. GW460-12-10-14-LB2)
MRVFLASIPLEAAKIVIIGGGEPALAKLRLFLNTPAEVVWFAPDGAPPKIETPLTAPEPV
LRAPEADELAGGRLVFMALDDADELTRLGHLARAAGAQVNVVDKPHLSDFQTPALIDRDE
VVIGVATGGSAPILARDIRTAIEGVLPAGLANVARLARELRDTVKASVPDFMARRRFWEK
AFRGPAATLAAEGRTAEARREMLRLLNTAAPEQGVVHIVGAGPGDPELLTLKALRVLQDA
DVIIHDRLVPEAVLERARRDAKRLYVGKARGEHSVPQDQIEALMIEEARAGHRVVRLKGG
DPFVFGRGGEELETMRAAGVPVFVVPGVTAALACAASAGIPLTHRDHAQAVTFVTAQAKP
GGVEADWTRLAGPNHTLAIYMGTDRAEETAARLIAAGRSPSTPVAIVENGSRPDERVLTG
HLDGLGAMVRGADLTGPALLFVGETAAFSAFAKTRTEAAA