Protein Info for A4249_RS09385 in Brevundimonas sp. GW460-12-10-14-LB2
Annotation: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 70% identical to ISPH_CAUVC: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) from Caulobacter vibrioides (strain ATCC 19089 / CB15)
KEGG orthology group: K03527, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [EC: 1.17.1.2] (inferred from 82% identity to bsb:Bresu_2726)MetaCyc: 54% identical to 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (Escherichia coli K-12 substr. MG1655)
RXN-24043 [EC: 1.17.7.4]; 1.17.7.4 [EC: 1.17.7.4]
Predicted SEED Role
"4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)" in subsystem Isoprenoid Biosynthesis or polyprenyl synthesis (EC 1.17.1.2)
MetaCyc Pathways
- superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (12/12 steps found)
- taxadiene biosynthesis (engineered) (12/13 steps found)
- methylerythritol phosphate pathway I (9/9 steps found)
- methylerythritol phosphate pathway II (9/9 steps found)
- isoprene biosynthesis I (9/10 steps found)
- superpathway of ergosterol biosynthesis II (11/26 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Terpenoid biosynthesis
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.17.1.2 or 1.17.7.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160HZY8 at UniProt or InterPro
Protein Sequence (323 amino acids)
>A4249_RS09385 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (Brevundimonas sp. GW460-12-10-14-LB2) MNAHVPVPNAPLRPIAVRLATPRGFCAGVDRAIQIVERAIEKFGAPVYVRHEIVHNKHVV ERLKAMGAVFVKELDECPDDRPVVFSAHGVPKSVPATARARELVFLDATCPLVSKVHVEA ERHHAAGRHIILIGHAGHPEVVGTLGQLPPGAITLVETEADAESFDRPGDAPLAYATQTT LSVDDTAGILKVLKRRFPELPDPHKEDICYATTNRQDAVKALGEGCDLVLVVGSKNSSNS VRLVEVAMRAGAPAAYLVDDASQVDWSWFDGVNTVGVTAGASAPEPLIEALVDAIRARFD ATVTEDDGARETVTFKLPRLLTA