Protein Info for A4249_RS09340 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: aminomethyl-transferring glycine dehydrogenase subunit GcvPB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 PF02347: GDC-P" amino acids 70 to 466 (397 residues), 37.4 bits, see alignment E=2.3e-13 PF00266: Aminotran_5" amino acids 173 to 300 (128 residues), 31.9 bits, see alignment E=1.1e-11 PF21478: GcvP2_C" amino acids 378 to 476 (99 residues), 80.5 bits, see alignment E=1.4e-26

Best Hits

Swiss-Prot: 82% identical to GCSPB_CAUVC: Probable glycine dehydrogenase (decarboxylating) subunit 2 (gcvPB) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K00283, glycine dehydrogenase subunit 2 [EC: 1.4.4.2] (inferred from 90% identity to bsb:Bresu_2715)

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P2 protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.4.2

Use Curated BLAST to search for 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NKT4 at UniProt or InterPro

Protein Sequence (519 amino acids)

>A4249_RS09340 aminomethyl-transferring glycine dehydrogenase subunit GcvPB (Brevundimonas sp. GW460-12-10-14-LB2)
MNTVGRPTAPNQVQTKPATLTGGRGLLQNEALIFEGDGWGKTGVDLPEPKTDGSDLGDLV
RKDPIGLPGLSEPETMRHYVRLSQKNHAIDLAIYPLGSCTMKHNPRLNEKMARLPGFSDI
HPLQPISTVQGALELMDTLAHWLKTLTGMPAVALSPKAGAHGELCGLMAIRAAHEASGQH
EKRRKVLVPTSAHGTNPATAAFVGYSVVEVAQTEDGRVDVADLASKLGDDVAAIMVTNPN
TCGLFERDILEISRLTHEAGAYFYCDGANFNAIVGRVRPGDLGVDAMHINLHKTFSTPHG
GGGPGAGPVVLSEALAPFAPAPWVVHDDGGYRLIEREEDEAEQAFGRLCAFQGQMGMYTR
ALSYMMSHGADGLRQVAEDAVLNANYIKARLGDLMSAAFPDGPCMHEALFDDEWLKGTDI
TTLDFAKAMIDEGFHPMTMYFPLVVHGAMLIEPTETESKAELDRFIEAMRALAEAAKAGD
VDRFKGAPFHAPLKRLDETRAARSPVLRWTAPEGSNKAA