Protein Info for A4249_RS09290 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: arginine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF03485: Arg_tRNA_synt_N" amino acids 3 to 87 (85 residues), 71.1 bits, see alignment E=1.4e-23 TIGR00456: arginine--tRNA ligase" amino acids 4 to 600 (597 residues), 407.5 bits, see alignment E=4.2e-126 PF00750: tRNA-synt_1d" amino acids 112 to 470 (359 residues), 275.3 bits, see alignment E=1.1e-85 PF05746: DALR_1" amino acids 485 to 600 (116 residues), 91.4 bits, see alignment E=6.5e-30

Best Hits

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 82% identity to bsb:Bresu_2724)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NKS6 at UniProt or InterPro

Protein Sequence (600 amino acids)

>A4249_RS09290 arginine--tRNA ligase (Brevundimonas sp. GW460-12-10-14-LB2)
MSDLKTVLSEAVAAAFAAEGMDPALARVTPSDRPDLADFQSNGALAAAKALKANPRELAG
KIAERLSADARFASVEVAGPGFINLKLSDALLAERAIEVANDADHAGAATVAAPRRVVID
YGGPNVAKPMHVGHLRSAIIGESLKRLFRFRGDHVTGDAHFGDWGFQMGLLIVACGDEGL
GEAFMAEGKGPFPAESPVTLADLDRLYPQAAGKAKEDPAFRDRARKATAELQNGRPGYRA
LWQHFVAVSREALKREYGDLSVDFDLWNGESDADPQMPEMLAHLKETGLLVEDDGAQVVH
VAKPGETRKKKLPDGSVIEAPSPPPLLVISSEGSAMYGTTDLATILDRKKALSPDLALYV
VDERQAEHFEQVFRAAYLAGYAEQGALEHLGFGTMNGQDGKPFKTRAGGVLKLRDLIDQA
TDKARERLHEAKLGDDLSPEEFEAIAHKVAIAALKFADLSNARTTSYVFDLDRFMSFEGK
TGPYLLYQSVRIKSLLRKGAEQGLTPGAIVIAEPAERDLALTLDAFSTAVSDAYDKRMPH
LVAEHAYSVAQSFSKFYAACPVLIAPDEATKRSRLALSAAALRQLEIALNLLGIDTPERM