Protein Info for A4249_RS09010 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: aminoacyl-tRNA hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 202 TIGR00447: aminoacyl-tRNA hydrolase" amino acids 2 to 170 (169 residues), 181.8 bits, see alignment E=5.4e-58 PF01195: Pept_tRNA_hydro" amino acids 3 to 177 (175 residues), 214.9 bits, see alignment E=4.4e-68

Best Hits

Swiss-Prot: 65% identical to PTH_PHEZH: Peptidyl-tRNA hydrolase (pth) from Phenylobacterium zucineum (strain HLK1)

KEGG orthology group: K01056, peptidyl-tRNA hydrolase, PTH1 family [EC: 3.1.1.29] (inferred from 86% identity to bsb:Bresu_2667)

Predicted SEED Role

"Peptidyl-tRNA hydrolase (EC 3.1.1.29)" (EC 3.1.1.29)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NKJ4 at UniProt or InterPro

Protein Sequence (202 amino acids)

>A4249_RS09010 aminoacyl-tRNA hydrolase (Brevundimonas sp. GW460-12-10-14-LB2)
MIIIAGLGNPGGKYEKNRHNIGFMAADEIARRWRFGPERAKFQSIVSEGLVDGTKVLLMK
PQTYMNESGRAVGEAARFYKIKPSEIIVFHDEIDLAPGRFRMKTGGGAAGQNGIRSLISH
LGADFRRARMGIGHPGEKELVMPHVLGDFHKAEQPWLDAMLQACADALPFAIAGDDERYQ
GEVMRLAPAPKFSPRQAARGEL