Protein Info for A4249_RS08810 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: thiamine phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00693: thiamine-phosphate diphosphorylase
  • amino acids 14 to 207 (194 residues), 173.9 bits, E=1.2e-55
• PF02581: TMP-TENI
  • amino acids 15 to 196 (182 residues), 191.1 bits, E=5.7e-61

Best Hits

Swiss-Prot: 66% identical to THIE_CAUSK: Thiamine-phosphate synthase (thiE) from Caulobacter sp. (strain K31)

KEGG orthology group: K00788, thiamine-phosphate pyrophosphorylase [EC: 2.5.1.3] (inferred from 70% identity to bsb:Bresu_1430)

Predicted SEED Role

"Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)" in subsystem Thiamin biosynthesis (EC 2.5.1.3)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• thiamine diphosphate biosynthesis I (E. coli) (2/2 steps found)
• thiamine diphosphate biosynthesis II (Bacillus) (2/2 steps found)
• thiamine diphosphate salvage II (4/5 steps found)
• thiamine diphosphate salvage V (2/3 steps found)
• superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
• thiamine diphosphate biosynthesis III (Staphylococcus) (1/3 steps found)
• thiamine diphosphate biosynthesis IV (eukaryotes) (1/3 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
• thiamine diphosphate salvage IV (yeast) (3/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (3/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.3

Use Curated BLAST to search for 2.5.1.3

Sequence Analysis Tools

See A0A160I267 at UniProt or InterPro

Protein Sequence (217 amino acids)

>A4249_RS08810 thiamine phosphate synthase (Brevundimonas sp. GW460-12-10-14-LB2)
MARTPTVPERPPCRLYLITPPVIADLNAFAQTLDQALAAGDVAALQIRLKPADDAALRTA
VEVLAPIARRHDVAVLLNDRPDLARATGCDGVHIGQEDGSLAEARRIMGPDAMIGVTCHD
DRDLAWDAAEGGADYVAFGAFYPTDTKETVHRPPLDILTVWQETVETPCVAIGGITVDNA
AELAQAGADFVAVSGGVWNHPDGAAEAVRRFNEKLRA