Protein Info for A4249_RS07835 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: phosphoenolpyruvate carboxykinase (GTP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 PF17297: PEPCK_N" amino acids 21 to 241 (221 residues), 333.5 bits, see alignment E=5.9e-104 PF00821: PEPCK_GTP" amino acids 245 to 601 (357 residues), 568.2 bits, see alignment E=5.7e-175

Best Hits

Swiss-Prot: 71% identical to PCKG_FRAAA: Phosphoenolpyruvate carboxykinase [GTP] (pckG) from Frankia alni (strain ACN14a)

KEGG orthology group: K01596, phosphoenolpyruvate carboxykinase (GTP) [EC: 4.1.1.32] (inferred from 84% identity to bsb:Bresu_1476)

MetaCyc: 56% identical to phosphoenolpyruvate carboxykinase [GTP], mitochondrial (Homo sapiens)
Phosphoenolpyruvate carboxykinase (GTP). [EC: 4.1.1.32]; 4.1.1.32 [EC: 4.1.1.32]

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [GTP] (EC 4.1.1.32)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.32)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HZC1 at UniProt or InterPro

Protein Sequence (615 amino acids)

>A4249_RS07835 phosphoenolpyruvate carboxykinase (GTP) (Brevundimonas sp. GW460-12-10-14-LB2)
MSILTTPGLRPAPTAHAGLIAWVEQIAALTKPAKVHWCDGSEAEKEAITADLLAKGTLKA
LDQTKRPGCYYAASDPRDVARVESRTFICSDDEADAGPTNNWADPIEMRARLDGLFDGCM
AGRTMYVVPFCMGPLGSEISALGVEITDSGYVALSMGVMTRMGKPALDQLGETGVFVPAV
HTLGAPLADGQADVAWPCNDDKWIVHFPETREIWSYGSGYGGNALLGKKCYALRIASIMA
RDEGWLAEHMLILKLTDPKGRAKYVAAAFPSACGKTNLAMLQPSLPGWKAETIGDDIAWM
RFGDDGRLYAVNPEAGFFGVAPGTSRNTNHNALETLASNTIFTNTALTNDGDVWWEGLTK
ETPEGLTDWKGRPHDPASGEPAAHPNARFAAPASQCPAIAPEWEDPRGVPIDAILFGGRR
ASAVPLVTEAFDWEHGVFMGSTVASEGTAAAENKVGELRRDPFAMLPFCGYNMGDYFDHW
LKMGQGRDASKLPRIFFVNWFRKDQDGRFVWPGFGDNARVLKWISERIDGEAEAVDTAVG
RVPSVAALDLSGLDLTPTQLSTLLDVDAGVWAEEAALIPDFYRQFGDRLPTALWDQHAAL
VARLDGAGAASMAAE