Protein Info for A4249_RS07680 in Brevundimonas sp. GW460-12-10-14-LB2
Annotation: FMN-dependent L-lactate dehydrogenase LldD
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 81% identical to LLDD_ECOK1: L-lactate dehydrogenase (lldD) from Escherichia coli O1:K1 / APEC
KEGG orthology group: K00101, L-lactate dehydrogenase (cytochrome) [EC: 1.1.2.3] (inferred from 81% identity to ecv:APECO1_2850)MetaCyc: 80% identical to L-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
1.1.5.M6 [EC: 1.1.5.M6]; RXN0-7227 [EC: 1.1.5.M6]
Predicted SEED Role
"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)
MetaCyc Pathways
- L-lactaldehyde degradation (aerobic) (2/2 steps found)
- methylglyoxal degradation IV (2/3 steps found)
- methylglyoxal degradation V (2/3 steps found)
- superpathway of methylglyoxal degradation (5/8 steps found)
- lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) (2/8 steps found)
- superpathway of fucose and rhamnose degradation (4/12 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.1.2.3 or 1.1.5.M6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160HZV8 at UniProt or InterPro
Protein Sequence (377 amino acids)
>A4249_RS07680 FMN-dependent L-lactate dehydrogenase LldD (Brevundimonas sp. GW460-12-10-14-LB2) MIISSPSDYREAARRKLPPFLFHYIDGGAYAEQTLRRNVEDWQAIALRQRVLQDMTSLSL ETKLFDETLRLPIILGPVGLTGMYARRGEVQAAKAAALRGVPFTMSTVSVCPIEEVAPAI DRPMWFQLYVLRDRGFMRNALERAKAEGVKTLVFTVDMPTPGARYRDAHSGMSGPNAEIR RMVQAMTHPAWAWDVGVRGTPHDLGNVSAYLGKPTGLADYIGWLANNFDPSISWKDLQWI RDFWDGPMIIKGILDPQDARDAVSFGADGIVVSNHGGRQLDGVLSSACALPAIANAVQGE LKILVDSGIRNGLDVVRAIALGADAVLLGRAFIYALAANGQAGVENLLDLFEKEMRVAMT LTGAKSVTGMTREMLAI