Protein Info for A4249_RS07250 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: NAD-dependent DNA ligase LigA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 777 PF01653: DNA_ligase_aden" amino acids 22 to 333 (312 residues), 373.7 bits, see alignment E=1.9e-115 TIGR00575: DNA ligase, NAD-dependent" amino acids 29 to 771 (743 residues), 745.1 bits, see alignment E=3.5e-228 PF03120: DNA_ligase_OB" amino acids 336 to 413 (78 residues), 113.3 bits, see alignment E=9.7e-37 PF03119: DNA_ligase_ZBD" amino acids 422 to 449 (28 residues), 39.8 bits, see alignment (E = 8.3e-14) PF00533: BRCT" amino acids 705 to 775 (71 residues), 49.1 bits, see alignment E=1.3e-16

Best Hits

Swiss-Prot: 58% identical to DNLJ_CAUVN: DNA ligase (ligA) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 76% identity to bsb:Bresu_1627)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NIA1 at UniProt or InterPro

Protein Sequence (777 amino acids)

>A4249_RS07250 NAD-dependent DNA ligase LigA (Brevundimonas sp. GW460-12-10-14-LB2)
MANAIPLKVPVEDLTAETAAEALAWLAAEMARHDALYNEATPEISDADYDALRARNLAIE
TAFPDLIRSDSPSNKVGAAASTQFAEVRHGVPMLSLDNAFDEGEVRDFVARITRFLKLPA
DEPIAFVAEPKIDGLSANLLYVDGKLTIGATRGDGRAGEDVTANLNTIADVKQTLAGDDW
PDRIEVRGEVYAPNDAFAAFNAAAEAEGRRTYANPRNFAAGSLRQKDARITAKRPLNFFA
YAWGEHSSDFAETQWDALQKLKAWGFPVNARSRRVEGAEGLIAVYRDLERDRATLGYDID
GVVYKVDRIDWQRRLGFVARSPRWAIAHKFPAQQATTVLEGIDIQVGRTGSLTPVARLHP
VTVGGVVVRNATLHNEDEIERLDVRIGDTVRLQRAGDVIPQILGVVPELRPADAAPYVFP
EHCPVCGSEAVREGDEVKRRCTGGLICDAQIVERLKHFVGRRAFDIEGLGEKQLVAFHAR
GWIKEPADIFRLARDETRLKELERDDGYGETSIRNLVAGIDARRIIALDRFLFGLGIRDI
GEQTSIVLARAFERWPALKAACLQAASGVPCDAWTDLAAAHAISPRVLAQMAEAKPPVVD
PWPEATMDMKISQAFPGMAAPARRSLATMVQDWSGLVELARVAREDGPSEILNQIAGVTG
VGPVAAEALAHFFHEPHNLRVVEALEAELTQILDAERPKSDTAVAGKTVVFTGALERFTR
DEAKARAESLGAKVSGSVSKKTDYLVAGPGAGSKLKDAEKHGVQVLTEDDWLALIGG