Protein Info for A4249_RS06225 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01853: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD
  • amino acids 17 to 331 (315 residues), 341.6 bits, E=1.9e-106
• PF04613: LpxD
  • amino acids 31 to 98 (68 residues), 62.2 bits, E=5e-21
• PF00132: Hexapep
  • amino acids 120 to 149 (30 residues), 27.7 bits, see alignment (E = 2.3e-10)
  • amino acids 133 to 166 (34 residues), 37.9 bits, 1.4e-13
  • amino acids 227 to 262 (36 residues), 37 bits, 2.7e-13
• PF14602: Hexapep_2
  • amino acids 134 to 166 (33 residues), 38.2 bits, 1.5e-13
  • amino acids 247 to 271 (25 residues), 12.9 bits, see alignment (E = 1.2e-05)
  • amino acids 264 to 294 (31 residues), 19.1 bits, see alignment (E = 1.3e-07)

Best Hits

Swiss-Prot: 42% identical to LPXD_GRABC: UDP-3-O-acylglucosamine N-acyltransferase (lpxD) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K02536, UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase [EC: 2.3.1.-] (inferred from 71% identity to bsb:Bresu_2489)

Predicted SEED Role

"UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase (EC 2.3.1.191)" (EC 2.3.1.191)

MetaCyc Pathways

• lipid IV_A biosynthesis (E. coli) (6/6 steps found)
• lipid IV_A biosynthesis (P. gingivalis) (8/9 steps found)
• superpathway of (Kdo)₂-lipid A biosynthesis (13/17 steps found)
• lipid IV_A biosynthesis (H. pylori) (5/6 steps found)
• lipid IV_A biosynthesis (P. putida) (5/6 steps found)
• lipid IV_A biosynthesis (Vibrio cholerae serogroup O1 El Tor) (5/6 steps found)
• lipid IV_A biosynthesis (generic) (5/6 steps found)
• lipid IV_A biosynthesis (2,3-diamino-2,3-dideoxy-D-glucopyranose-containing) (4/6 steps found)
• superpathway of Kdo₂-lipid A biosynthesis (13/25 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Anthocyanin biosynthesis
• Benzoate degradation via CoA ligation
• Biosynthesis of terpenoids and steroids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Carotenoid biosynthesis - General
• Diterpenoid biosynthesis
• Ether lipid metabolism
• Ethylbenzene degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Histidine metabolism
• Limonene and pinene degradation
• Lipopolysaccharide biosynthesis
• Lysine degradation
• Phenylalanine metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.- or 2.3.1.191

Sequence Analysis Tools

See A0A168NGT2 at UniProt or InterPro

Protein Sequence (337 amino acids)

>A4249_RS06225 UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (Brevundimonas sp. GW460-12-10-14-LB2)
MPDPRFFETLSPLSVADLAAQIGGEVDRGGDRMIASVAPLSSADGGAIAFLGDRKFAAAL
ATTQAGCVIVPAEAKDAAPSSAAVIISRTPQAAWAKASLALHRPILLEKAITRDEAGEDD
SVVVEPGAILGQGVRIGRGSRIGANSVIAPGVQIGRDCVIGANVSVAFALIGDRVKLYAG
ARIGEAGFGATGTAAGVMDIPQLGRVILQDGVTVGANSCIDRGAYDDTVIGENTKIDNLV
MVGHNCVIGRNCLLVANTGISGSVTVGDNVIFGGKAGIGDHITIGEGARVAAGAGVLADV
PAGETWSGYPARPIRQFLRETVWLAKQASSKKAPKES