Protein Info for A4249_RS06195 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: citrate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01798: citrate (Si)-synthase
  • amino acids 18 to 426 (409 residues), 746.7 bits, E=2.8e-229
• PF00285: Citrate_synt
  • amino acids 51 to 415 (365 residues), 456.8 bits, E=2.6e-141

Best Hits

Swiss-Prot: 72% identical to CISY_RHIME: Citrate synthase (gltA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01647, citrate synthase [EC: 2.3.3.1] (inferred from 88% identity to bsb:Bresu_2147)

MetaCyc: 67% identical to citrate synthase monomer (Acetobacter aceti)
Citrate (Si)-synthase. [EC: 2.3.3.1, 2.3.3.16, 2.3.3.3]

Predicted SEED Role

"Citrate synthase (si) (EC 2.3.3.1)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 2.3.3.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• mixed acid fermentation (10/16 steps found)
• methylaspartate cycle (12/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.1 or 2.3.3.16 or 2.3.3.3

Sequence Analysis Tools

See A0A168NGS4 at UniProt or InterPro

Protein Sequence (433 amino acids)

>A4249_RS06195 citrate synthase (Brevundimonas sp. GW460-12-10-14-LB2)
MTEQAKPAGSATLSFEDKTIELPVLSGSTGPDVIDIRKLYAGTGAFTFDPGFTSTAACES
ALTYIDGDAGVLLHRGYPIDQLASKSNFVEVCHLLLHGELPTAAQYEKFENSITTHTMLH
SQFDRFFEGFRRDAHPMAIMVGTVGALSAFYHDSLDIHDPIQRDISAIRLIAKMPTIAAR
AYKYHIGQPFVSPRNDLSYAENFLRMCFAVPAEDYHVDPALVRAMDRIFTLHADHEQNAS
TSTVRLAGSSGANPFACIAAGIACLWGPSHGGANEEALTMLKEIGTPDKIPEFIQGVKDR
KYKLMGFGHRVYKNYDPRAKVMQQSAYEVLAATGRENDPLFLVAKELEKVALSDEYFTSR
KLFPNVDFYSGITLSAMGFPTTMFTVLFALARTVGWISQWQEMMADPSQKIGRPRQLYTG
PTQRDYVPIEQRG