Protein Info for A4249_RS05575 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: DNA topoisomerase IV subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 694 PF02518: HATPase_c" amino acids 80 to 223 (144 residues), 63.2 bits, see alignment E=5.8e-21 PF00204: DNA_gyraseB" amino acids 301 to 449 (149 residues), 129.9 bits, see alignment E=1.4e-41 PF01751: Toprim" amino acids 477 to 584 (108 residues), 63.1 bits, see alignment E=4.7e-21 PF00986: DNA_gyraseB_C" amino acids 620 to 682 (63 residues), 81.9 bits, see alignment E=5.9e-27

Best Hits

Swiss-Prot: 73% identical to PARE_CAUVC: DNA topoisomerase 4 subunit B (parE) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 83% identity to bsb:Bresu_1865)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HZ07 at UniProt or InterPro

Protein Sequence (694 amino acids)

>A4249_RS05575 DNA topoisomerase IV subunit B (Brevundimonas sp. GW460-12-10-14-LB2)
MAADLFAFDDEPEARKPEPPKPVVVTPPAAAPTAVSTPAPPPVQSTATTAAATGYSASSI
EVLEGLEPVRKRPGMYIGGTDERALHHLFAEVLDNAMDEAVAKHAKLITVDLDADGYLSV
RDDGRGIPVDPHPKHPGKSALEVVMTVLHSGGKFSGKAYETSGGLHGVGVSVVNALSESV
EVTVWRDGFEWKQAFSRGHVLAPIQQIGPSKKRGTLIRFKPDDEIFGMGAAFKPARLFRM
ARSKAYLFRGVEIRWTCAPERITDATPAQASLHFPGGLADALIDRIGQLETVTPAFAGRA
ERQGEAGAFEWAVTWSPIGFGESDGFIQSYCNTVSTPDGGTHEAGFRAALVKGLKSYGEL
TNEKRAAIITAEDVIANAGALISVFIRNPEFQGQTKDRLSSPEGARIVEQLLRDPLDHWL
TESPKQANALLGFVVDRAEDRLRRRKDKEVQRAAATRKLRLPGKLSDCSRQSAQGTELFI
VEGDSAGGSAKQARDRTTQAILPLRGKILNVASATADKLRANVELSDLALALGVQPGNRF
NIDDLRYERIVIMTDADVDGAHIAALLITFFYRVMPETIRQGRVFMALPPLYRISAGPLS
EYARDDAHRDELLATVFKGKKTEIGRFKGLGEMMASQLKETTMDPKKRTLARITVPDAEA
SIEDLVERLMGKRADARFQFIQENAQFVKEELDV