Protein Info for A4249_RS05505 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 719 PF13328: HD_4" amino acids 26 to 175 (150 residues), 151.8 bits, see alignment E=4.1e-48 TIGR00691: RelA/SpoT family protein" amino acids 26 to 713 (688 residues), 660.1 bits, see alignment E=1.9e-202 PF01966: HD" amino acids 45 to 144 (100 residues), 34.5 bits, see alignment E=7e-12 PF04607: RelA_SpoT" amino acids 235 to 344 (110 residues), 142.7 bits, see alignment E=1.7e-45 PF02824: TGS" amino acids 394 to 452 (59 residues), 76 bits, see alignment 5.8e-25 PF19296: RelA_AH_RIS" amino acids 465 to 555 (91 residues), 59.5 bits, see alignment E=1.5e-19 amino acids 571 to 626 (56 residues), 42.6 bits, see alignment 2.2e-14 PF13291: ACT_4" amino acids 643 to 715 (73 residues), 58.7 bits, see alignment E=2.2e-19

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 86% identity to bsb:Bresu_2507)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HWG0 at UniProt or InterPro

Protein Sequence (719 amino acids)

>A4249_RS05505 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Brevundimonas sp. GW460-12-10-14-LB2)
MLRQFELIEAVKAYDPTADEALLNRAYVYAMKMHGSQLRASGDPYFAHPIQVAGILTDYK
LDTATIVTALLHDVVEDTSATRDDIAGMFGEEVAVLVEGVTKLSRLELQAEHTRQAENLR
KFILAISRDVRVLLVKLADRLHNMRTLQFVKPEKRERISRETLEVYAPLGRSIGIHSIAS
ELEELAFTHLNPTAKTAIERRLEALKLEHGRAIDGVASEVERVLSEAGLKANVYGRQKTP
YSIWRKLQRKSVGFSSLSDIYGFRVIVEAEDDCYRALGVIHREWPMVPERFKDFISTPKS
NNYRSLHTTVVGPRGLRIEMQIRTEDMDRIAEDGVAAHWRYKNHSYGFDREAMEQGGGRD
PLQNLRHLVQVIEHGEGGEDWVEHAKLEMYLDQVFVFTPKGQLVTLPRGGMPLDFAYAVH
TEVGDTAVGVKVNGELKPMRTPLQNGDVVEIIRGSKREVPADWRSLTVTGRARSAIRRHI
RTSEREEFIKLGRVALDQTLGRVDKALTDVSLKPVLELLAVASDDDLFEGLGRGRLSPTT
AAEILFPALKGRLKAGPEKQRIEGEKARLFVRGGGLTPGVSVHFGQCCTPVPGDRIVGIL
EPESGLTVHTIDCQTLAAFADDDSVWHDLQWTPQAETDAVAAARIRATIRNAPGVLGQVT
TLIGEAGGNIINLSMAHRQQDFFDVDVDVEVEDAKHATMIMAALRANPSVDSVERARGE