Protein Info for A4249_RS05230 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: DNA polymerase IV

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 PF00817: IMS" amino acids 12 to 158 (147 residues), 188.4 bits, see alignment E=1e-59 PF11798: IMS_HHH" amino acids 171 to 201 (31 residues), 30.6 bits, see alignment (E = 4e-11) PF11799: IMS_C" amino acids 244 to 354 (111 residues), 89.7 bits, see alignment E=2.6e-29

Best Hits

Swiss-Prot: 63% identical to DPO42_RHIME: DNA polymerase IV 2 (dinB2) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02346, DNA polymerase IV [EC: 2.7.7.7] (inferred from 78% identity to bsb:Bresu_1659)

Predicted SEED Role

"DNA polymerase IV (EC 2.7.7.7)" in subsystem DNA repair, bacterial (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HYA5 at UniProt or InterPro

Protein Sequence (357 amino acids)

>A4249_RS05230 DNA polymerase IV (Brevundimonas sp. GW460-12-10-14-LB2)
MSGVATRKIVHVDMDAFFASVEQRDDPSLRGRPVAVGHAAARGVVAAASYEARRFGVRSA
MPSTTAQRKCPDLVFVPPRFDVYRAVSAQIHAIFADYTDLIEPLSLDEAYLDVTADRRIL
GTASATAEEIRRRILEETGLTASAGISYNKFLAKLASDQNKPNGQFVVAPGRGEAFVEDL
PVRRFHGVGPVTAAKMEKLGIRTGADLRRQPLAFLQHHFGKSGPWYYAIARGEDDRPVNP
DRTRKSSGSETTFASDLTEPEAIEAGVVAMADEVWAWCEIAGSRGRTVTVKVKWADFQQS
TRSRSLSEPVASREALRDLSRLLVRSLYPPTRGVRLVGVSLSNLETTGAAANQLALS