Protein Info for A4249_RS04820 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: sigma-54 dependent transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 PF00072: Response_reg" amino acids 8 to 119 (112 residues), 99.6 bits, see alignment E=4.2e-32 PF00158: Sigma54_activat" amino acids 145 to 308 (164 residues), 172.5 bits, see alignment E=2.2e-54 PF14532: Sigma54_activ_2" amino acids 146 to 313 (168 residues), 94.6 bits, see alignment E=2.3e-30 PF07728: AAA_5" amino acids 169 to 280 (112 residues), 24.9 bits, see alignment E=6.2e-09 PF02954: HTH_8" amino acids 414 to 452 (39 residues), 37 bits, see alignment 8e-13

Best Hits

Swiss-Prot: 65% identical to NTRX_AZOC5: Nitrogen assimilation regulatory protein NtrX (ntrX) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K13599, two-component system, NtrC family, nitrogen regulation response regulator NtrX (inferred from 86% identity to bsb:Bresu_1783)

Predicted SEED Role

"Nitrogen regulation protein NtrX"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HW03 at UniProt or InterPro

Protein Sequence (468 amino acids)

>A4249_RS04820 sigma-54 dependent transcriptional regulator (Brevundimonas sp. GW460-12-10-14-LB2)
MRPTGSDILVVDDEADIRDLVSGLLEDEGHAVRVAANSDEALAAIRARKPSLAILDIWMQ
GGGLDGLELLEVVKELDPDLPVVMISGHGNIETAVTALKRGAYDFIEKPFKSDRLVVVVE
RAIEAATLRRENRRLRAATMTPSGLIGRSQAAQALRSTIAKVAQANSRVLIAGPAGSGKE
LVARQIHEASPRARAEFVAISAAGMTPERLDVELFGEEGQDGRPRKIGVFERAHNGTLYL
DEISDMPRESQSRILRVLVDQRFRRVGGEQDVQVDVRVVTSTSRDLKAEIAEGRFREDLF
HRLNVVPIRVPPLSERREDIAELVEYFVDTISASQGLPKRIVGDDAVAVLQVHPWPGNIR
QLRNNIERLLILATGDPAETITADMLPQEVATSNGGTANLGGERTIALPLREAREVFERE
YLAAQIMRFGGNISRTAAFIGMERSALHRKLKSLGVSPARGGDEEEPA