Protein Info for A4249_RS04230 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: type I DNA topoisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 868 TIGR01051: DNA topoisomerase I" amino acids 3 to 636 (634 residues), 760.7 bits, see alignment E=9.1e-233 PF01751: Toprim" amino acids 3 to 115 (113 residues), 77.9 bits, see alignment E=1.1e-25 PF01131: Topoisom_bac" amino acids 130 to 555 (426 residues), 420.9 bits, see alignment E=1.2e-129 PF01396: zf-C4_Topoisom" amino acids 601 to 636 (36 residues), 50.9 bits, see alignment (E = 2.2e-17) PF13368: Toprim_C_rpt" amino acids 656 to 712 (57 residues), 54.2 bits, see alignment 2.2e-18 amino acids 716 to 766 (51 residues), 62.9 bits, see alignment 4.4e-21 amino acids 784 to 833 (50 residues), 75.9 bits, see alignment 3.8e-25

Best Hits

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 81% identity to bsb:Bresu_1829)

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HW04 at UniProt or InterPro

Protein Sequence (868 amino acids)

>A4249_RS04230 type I DNA topoisomerase (Brevundimonas sp. GW460-12-10-14-LB2)
MNLVIVESPAKAKTINKYLGSDYEVLASYGHVRDLPSKDGSVLPDDDFSMHWEADAKGAK
RLSEIAEAAKRADRVILATDPDREGEAISWHVLEVLNKKKALKDTHVERVTFNAITKSAV
LEAMANPRQIDMELVEAYLARRALDYLVGFTLSPVLWRKLPGARSAGRVQSVALRIVVDR
EMEIEKFKAQEYWSIEADLNADSPPFTARLVKHAGKRIQRLDIKDEATASAARAAIAAGD
FTINAVEKKPVRRNPAPPFTTSTLQQEAARKLGFSAQRTMQAAQKLYEGVDETGGLITYM
RTDGVQTTPEGIAQAREVISQAFGPAFVPQEPRYYKTKAKNAQEAHEAIRPTNIARHPDS
LRLESDLQRLYELIWKRMVASQMEAARMDRTTVDIETTDGQTGLRATGQVVTFDGFLAVY
EEGRDEKQKGAEDDESDDTSRLPALKEGAKAKVDAIRTDQHFTEPPPRYSEATLVKKLEE
LGIGRPSTYASTLSTLRDREYVRVDKNRFYPEDKGRLVTAFLEQFFKKWVEYDFTAALET
QLDEVSAGQLDWKVLLRNFWQDFHAATQAAGELRTTAILDALNESLGAHIFPDKGDGTDP
RECPLCHQGQLSLKTSRFGAFIGCDRYPECKYTRPVASPSAEDGSAESGDRELGIDPETG
QPVQLKIGRFGPYVEITPPEGDKPKRSSLPKDWSPASLSLDQALRLLALPREVGVHPEDG
KMITAGLGRYGPFVLHAGTYANVSDIDEVFEVGLNRAVALLAEKRAGRPGRGAATAPLKD
LGAHPETGESIHVMAGRFGPYVKSGKINATLPKGTAPEDLTLEDALPLLAAKAGAAPKKK
APAKKAAAKKPAAKKAPAKKAAKSKAAD