Protein Info for A4249_RS04200 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 517 PF01479: S4" amino acids 97 to 143 (47 residues), 40.9 bits, see alignment 1.4e-14 PF00849: PseudoU_synth_2" amino acids 160 to 298 (139 residues), 54.6 bits, see alignment E=1.5e-18 TIGR00093: pseudouridine synthase" amino acids 162 to 328 (167 residues), 127.5 bits, see alignment E=2e-41

Best Hits

KEGG orthology group: K06178, ribosomal large subunit pseudouridine synthase B [EC: 5.4.99.12] (inferred from 63% identity to bsb:Bresu_2386)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70)" in subsystem Two cell division clusters relating to chromosome partitioning (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160I2H0 at UniProt or InterPro

Protein Sequence (517 amino acids)

>A4249_RS04200 pseudouridine synthase (Brevundimonas sp. GW460-12-10-14-LB2)
MVRHTDDNDKKPRARQDERSPRPFKSSGPRKSGDGTRSFGDKPRGPREDGAMRFGDKADR
PRSDKPRSDRPRTDKPRSDGGGKDGKSKTPDTPLRAERIAKTMARAGIASRREVERLIGL
GKVAVNGRILDTPATLVTRDDVITVDGKPIGSTQATRVWRYHKPAGLLTSHSDPTGRPTV
FDALPAGLPRVISVGRLDLATEGLLLLTNDGELSRALELPSTSLVRQYRARARGKITQEQ
LDALKDGVVVDGVSYGPIEAKLDKAKESKSEDGKAPANLWISVSITEGKNREVRKVLESV
GLTVNRLIRLAYGPFRLDVLPIGSVEEVGPRVIRELLADYIRPENLPTGNTVETPAPIPG
RRVSTPIVKGRSGSAMSDPSRKPSRVRAAETAQADAVERRERPPKKEGWAKAAPKFEHSK
SFKPRERTAPAGDRPAREDGDRPKRAFKPRDDQFIDDRRGKPGAKPAGRAGSGPRAGGPG
GKPAGGRDFKPRTGGGKPAGPSGTSGRGPGGKPRTPR