Protein Info for A4249_RS04120 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: cisplatin damage response ATP-dependent DNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 562 TIGR04120: DNA ligase, ATP-dependent, PP_1105 family" amino acids 181 to 549 (369 residues), 594.5 bits, see alignment E=1.2e-182 PF01068: DNA_ligase_A_M" amino acids 225 to 419 (195 residues), 109.9 bits, see alignment E=2e-35 PF04679: DNA_ligase_A_C" amino acids 440 to 534 (95 residues), 40.4 bits, see alignment E=4.9e-14

Best Hits

KEGG orthology group: K01971, DNA ligase (ATP) [EC: 6.5.1.1] (inferred from 82% identity to bsb:Bresu_2256)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) LigC" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.1

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160I213 at UniProt or InterPro

Protein Sequence (562 amino acids)

>A4249_RS04120 cisplatin damage response ATP-dependent DNA ligase (Brevundimonas sp. GW460-12-10-14-LB2)
MRAFAELLDRLSLTASRNAKLVLVRDYLRATPDPDRGWALAALTGDLTFDAAKPAMIRKA
VEGRVDSVLFGWSYDYVGDLAETVALIWPVTPDHRPNREPELAEVVEALRTATRTEVRGL
LESWLDALEPKGRWALLKLMTGALRVGLSSRLAKTAAAMMRPDAVMAPPSPEGDEEQMTL
EALDASAIEEVWHAVEPPYADLFAWLEGRAERPSPDAPGRFRPVMLAVAIDEPVDLPKLS
PADYAAEWKWDGIRVQAVLEGEVRKLYSRTGDEISAAFPDVMEALAFQGAIDGELIVWRD
GAVAPFGDLQQRLNRKSVDAKAMQAFPAAVVAYDLLAQDGEDLRGLPLRERRARLEALVN
GHTGERLHLSPVVDYADWDQLARLRADPPVGAAAEGLMLKRWDSPYLAGRPKGPWFKWKR
DPHVIDAVLMYAQRGHGKRSSFYSDYTFGVWTPEGALTPVGKAYFGFTDEELKQLDKFVR
DHTIDRYGPVRSVRAERDFGLVLEIAFEGLNRSTRHKSGVAMRFPRVSRIRWDKPAREAN
TIDDVMDLLDAIESGGGRIAKA