Protein Info for A4249_RS03345 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: type II restriction endonuclease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 PF27126: NmeDI_C" amino acids 247 to 399 (153 residues), 117.7 bits, see alignment E=2.4e-38

Best Hits

KEGG orthology group: None (inferred from 74% identity to npp:PP1Y_AT24187)

Predicted SEED Role

"Putative type II restriction enzyme NmeDIP (EC 3.1.21.4) (Endonuclease NmeDIP) (R.NmeDIP)" (EC 3.1.21.4)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HXJ8 at UniProt or InterPro

Protein Sequence (423 amino acids)

>A4249_RS03345 type II restriction endonuclease (Brevundimonas sp. GW460-12-10-14-LB2)
MPDRLRDLIAQWRNDPSATYQTWFLWDERLKNFRSIGRGIGQVVEEIETGRFGVAYRGSS
LETIVHSVAEQRQIFKGADHAFLWKPKLRIPDIYENPANQRAFGRLLHHCSGCSTADEIL
SGIRTIDQLKIKGLGPAVANLIYFLHPTHAPPFNTAIVNGYNAITGSKVKLGSWEHYLAM
REGLLALNARYRDLLSNDLGALAGFLFDVGSGRYPAPPPAGDALSRQGWEERLAQARAEA
LKFEKATAQQRETDRTHTEIQAWLRDLGRALGYRVWIAANDRSRLHDGARLGEGCLDQLP
TALANGAGAESIRLIDVLWLDASPDRVAAAFEVEHSTTIYSGVVRMLDLALSGELHTSAG
LFLVAPDSREGDVRAQLQRPAFSRISDLDVRYLPYGELDRHRGSIARFGSDLRGLTEISH
RLT