Protein Info for A4249_RS03270 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 784 transmembrane" amino acids 130 to 151 (22 residues), see Phobius details amino acids 163 to 181 (19 residues), see Phobius details amino acids 193 to 218 (26 residues), see Phobius details amino acids 232 to 251 (20 residues), see Phobius details amino acids 387 to 408 (22 residues), see Phobius details amino acids 414 to 437 (24 residues), see Phobius details amino acids 730 to 749 (20 residues), see Phobius details amino acids 755 to 777 (23 residues), see Phobius details PF04945: YHS" amino acids 27 to 71 (45 residues), 42.6 bits, see alignment 1.2e-14 PF19335: HMBD" amino acids 85 to 111 (27 residues), 50.8 bits, see alignment (E = 3.2e-17) TIGR01511: copper-translocating P-type ATPase" amino acids 177 to 780 (604 residues), 602.3 bits, see alignment E=2e-184 TIGR01525: heavy metal translocating P-type ATPase" amino acids 195 to 779 (585 residues), 633.5 bits, see alignment E=7.9e-194 TIGR01512: cadmium-translocating P-type ATPase" amino acids 236 to 779 (544 residues), 406 bits, see alignment E=4.5e-125 TIGR01494: HAD ATPase, P-type, family IC" amino acids 241 to 748 (508 residues), 288.6 bits, see alignment E=1.4e-89 PF00122: E1-E2_ATPase" amino acids 269 to 450 (182 residues), 187 bits, see alignment E=6.1e-59 PF00702: Hydrolase" amino acids 467 to 684 (218 residues), 127.6 bits, see alignment E=2.1e-40

Best Hits

Swiss-Prot: 63% identical to SILP_SALTM: Silver exporting P-type ATPase (silP) from Salmonella typhimurium

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 82% identity to bsb:Bresu_1670)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168ND63 at UniProt or InterPro

Protein Sequence (784 amino acids)

>A4249_RS03270 heavy metal translocating P-type ATPase (Brevundimonas sp. GW460-12-10-14-LB2)
MATSSHSHPAGHGCCSNKTPQAGAQAIDPVCGMTVDPATTAHGAAHAGEEYVFCSAGCRT
KFVDDPDRYLGELPEPAPVTPGAIYTCPMHPEVRQEGPGSCPICGMALEPETVTAEAPVN
HELIDFTRRLWIGAVLTLPVFALEMGGHLTGLAMRIPGQTSNWIQFALATPVVLWSGWPF
FQRGWASIRNRSLNMFTLIAIGVGAAWIYSVVAVLAPHLFPAAVRRMDGSAPVYFEAAAI
ITVLVLVGQILELRAREQTSGAIRALLDLAPKTARRVRNDGGDEDVTLDLVAVGDRLRVR
PGEKVAVDGEILDGRVTLDESLVTGESMPVTKDVGDKVVAGSLNKTGSFVMRADKVGADT
LLAQIVQMVAQAQRSRAPIQRLADKVSGWFVPAVIGIAVLAAIVWGLAGPEPRLSYALVA
AVSVLIIACPCALGLATPISIMVGVGRGAHAGVLIKNAEALERFEKVDTLVLDKTGTLTE
GRPSVTAVRPADGFDEAELLRLSASLERSSEHPLADAVVRAASDQNLVLSEASDFDSPVG
RGVTGVVDGRRVALGNSRYLAEIGVDVGPLEAGADTLRQDGATAIFVAVDGAAAGVLGIA
DPVKATTAEAIRDLKASGLRLVMMTGDNRTTAEAVARRLGIDDVQAEVLPQDKAAVVERL
RAEGRIVAMAGDGVNDAPALAAADVGVAMGAGSDVAIESAGVTLLGGDLQGLVRARKLSR
AVMSNIRQNLVFAFGYNTLGIPIAAGLLYPVSGLLLSPALAALAMALSSVSVIANALRLR
AVRL