Protein Info for A4249_RS02965 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: cyclopropane-fatty-acyl-phospholipid synthase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 341 PF02353: CMAS" amino acids 62 to 300 (239 residues), 142.3 bits, see alignment E=6.8e-45 PF05175: MTS" amino acids 112 to 223 (112 residues), 41 bits, see alignment E=5.8e-14 PF13489: Methyltransf_23" amino acids 113 to 236 (124 residues), 56.7 bits, see alignment E=9e-19 PF13847: Methyltransf_31" amino acids 115 to 229 (115 residues), 45.4 bits, see alignment E=2.6e-15 PF13649: Methyltransf_25" amino acids 120 to 214 (95 residues), 57.3 bits, see alignment E=7.7e-19 PF08241: Methyltransf_11" amino acids 121 to 218 (98 residues), 45.7 bits, see alignment E=3.1e-15 PF08242: Methyltransf_12" amino acids 121 to 215 (95 residues), 44.4 bits, see alignment E=7.9e-15

Best Hits

KEGG orthology group: K00574, cyclopropane-fatty-acyl-phospholipid synthase [EC: 2.1.1.79] (inferred from 80% identity to bsb:Bresu_1722)

MetaCyc: 45% identical to phenylalkylamine N-methyltransferase (Ephedra sinica)
2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]

Predicted SEED Role

"Cyclopropane-fatty-acyl-phospholipid synthase-like protein, clusters with FIG005069"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.79

Use Curated BLAST to search for 2.1.1.79 or 2.1.1.M76

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NCU4 at UniProt or InterPro

Protein Sequence (341 amino acids)

>A4249_RS02965 cyclopropane-fatty-acyl-phospholipid synthase family protein (Brevundimonas sp. GW460-12-10-14-LB2)
MNLTNVAIRQLQDAPFPDFVTRPAIDSLVTEARKRLDRDGPHDEAAFAREMAQRAIAEHT
DAANQQHYELPPEFFQLCLGKRLKYSCCLYPTGRETLDQAEEAALAETCAHADLKDGLTI
LEMGCGWGSLSLWMAETYPKSKITAVSNSHGQRGHIEAQAAARGLTNLTVITQDMNAFDT
EQRFDRIVSVEMFEHMANWRALLTKARNWLTPDGRMFIHVFTHRDAPYRFDHTDSGDFIA
QHFFTGGVMPSHGLIRQFPDLFAVEQEWTWNGGHYAKTANDWLANMDRNAPRIAVLMRET
YGDDAKLWTRRWRRFYLATAGLFGNDEGRTWAVSHYRLKPV