Protein Info for A4249_RS02795 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 925 TIGR01063: DNA gyrase, A subunit" amino acids 22 to 906 (885 residues), 1078.5 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 46 to 511 (466 residues), 512.1 bits, see alignment E=1.3e-157 PF03989: DNA_gyraseA_C" amino acids 543 to 589 (47 residues), 50.5 bits, see alignment 1.1e-17 amino acids 595 to 642 (48 residues), 12.3 bits, see alignment 9.4e-06 amino acids 653 to 696 (44 residues), 22.4 bits, see alignment 6.8e-09 amino acids 700 to 746 (47 residues), 43.9 bits, see alignment 1.3e-15 amino acids 809 to 838 (30 residues), 29.8 bits, see alignment (E = 3.3e-11) amino acids 860 to 905 (46 residues), 45.3 bits, see alignment 4.7e-16

Best Hits

Swiss-Prot: 55% identical to GYRA_GRABC: DNA gyrase subunit A (gyrA) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 88% identity to bsb:Bresu_2628)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NWC7 at UniProt or InterPro

Protein Sequence (925 amino acids)

>A4249_RS02795 DNA gyrase subunit A (Brevundimonas sp. GW460-12-10-14-LB2)
MTDDSYENAASPINGRGGGSDISTITIEDELKRSYLDYAMSVIVSRALPDARDGLKPVHR
RILYSMHDLNMTPERSYSKCARVVGDVLGRFHPHGDASVYMALVRMAQPFSMGLMLVDGQ
GNFGSVDGDMPASMRYTEARMAPAAVALMADIDKDTVDFQPNYDEKELEPVVLPSRIPNL
LVNGAGGIAVGMATNIPPHNLGEVVDAALALLDDPNVTDDALLDIVPGPDFPTGGEIMGR
TAPRNALRDGRGSVVVRGRASVEEIRKDREAIVVTELPYQVNKQTLIERIAEMVREKRLE
GISDVRDESDRQGMRIVIELKRDASGDVILNQLWRYTAMQSSFGVNMLALNHGRPEQMGL
RQLLQIFLDFREEVVVRRVKFELNKARDRGHVLVGLAVAVANIDEVIHIIRSSADPTEAR
ERLQAKAWPVGDMMALVELIADPRTVIVEGDKIRLTDEQARAILALTLSRLTGLGRDDIF
GEAHGLADTIQGHLTILSDRKNVLAIIRDDLIDVKDRFAVPRRTLIGEGDGEMEDEDLIP
REDMVVTVTHGGYVKRVALNAYRTQHRGGKGRSGMSMKDEDAITGVFSASTHTPVLFFAT
NGKAYKLKTWRLPLGNPQSRGKAFVNLLPIEPGDSIMNVLPLPEDETTWGDYDIMFATSS
GDVRRNKLSDFATVNRAGKIAMKLEGSDRMVGVGLCTADDDVLLTTALGRAIRFKADDVR
VFKGRDSTGVRGVRLQDGDEVISMAILGRVDATPEERAAYVKHANAMRKALAGAEAEEDA
AAPVEAEDEVADAALTVERIAELGAAEQFILTVTETGFGKRSSAYEYRRTGRGGQGLTAH
GLGGRAGTRLAAAFPVEETDDLLLVTSGGQMIRTRIDQVRIVGRSSQGVTIFRTGKDEKV
VSVERLPESGGADDADVAGEEGSEA