Protein Info for A4249_RS02515 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: DNA topoisomerase IB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 PF21338: Top1B_N_bact" amino acids 36 to 84 (49 residues), 95.6 bits, see alignment 1.1e-31 PF01028: Topoisom_I" amino acids 100 to 306 (207 residues), 128.3 bits, see alignment E=3.1e-41

Best Hits

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 75% identity to bsb:Bresu_2737)

Predicted SEED Role

"DNA topoisomerase IB (poxvirus type) (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NA60 at UniProt or InterPro

Protein Sequence (340 amino acids)

>A4249_RS02515 DNA topoisomerase IB (Brevundimonas sp. GW460-12-10-14-LB2)
MADGSHAFDIAAEVPQGLSYCSDEFAGLTRRRAGKGWSYRDAKGKTVTDAKTLARIRALA
IPPAWTDVWICPKANGHIQATGRDVKGRKQYRYHNDWSTARSENKFDKLPAFSRNLPKLR
EKVEHDLALRGVCRDKVLATAVRLLEITLIRVGNSIYAKQNRSYGLTTLHKRHIDVDGAA
LTFEFRGKSGKDHSVSVKDRRLAKVVRQLEGLPGQQLFKYRAADGALCPVDSDDVNAYIR
EAMGEQFSAKDFRTWAGTVSAARALRDTEAPTSPTDAKRKITVCVKAVAGLLGNTPTVCR
ASYVHPKVFALFESGEIGRALPGSETKGFEKALIKQLLAV