Protein Info for A4249_RS02510 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: L-threonine 3-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 PF08240: ADH_N" amino acids 29 to 139 (111 residues), 94.5 bits, see alignment E=5.4e-31 PF16912: Glu_dehyd_C" amino acids 158 to 335 (178 residues), 41.2 bits, see alignment E=2.1e-14 PF00107: ADH_zinc_N" amino acids 179 to 309 (131 residues), 83.8 bits, see alignment E=1.5e-27

Best Hits

Swiss-Prot: 67% identical to TDH_RUEPO: L-threonine 3-dehydrogenase (tdh) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K00060, threonine 3-dehydrogenase [EC: 1.1.1.103] (inferred from 94% identity to bsb:Bresu_2739)

MetaCyc: 57% identical to threonine dehydrogenase (Escherichia coli K-12 substr. MG1655)
L-threonine 3-dehydrogenase. [EC: 1.1.1.103]

Predicted SEED Role

"L-threonine 3-dehydrogenase (EC 1.1.1.103)" in subsystem Glycine Biosynthesis or Threonine degradation (EC 1.1.1.103)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.103

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NA45 at UniProt or InterPro

Protein Sequence (350 amino acids)

>A4249_RS02510 L-threonine 3-dehydrogenase (Brevundimonas sp. GW460-12-10-14-LB2)
MTDTMKALAKTAPGVGLELIDAPIPEAGPEDVLIRVHRTAVCGTDIHIWNWDEWSQKNVP
TPMITGHEFAGEIVAIGKDVDRPLKIGQRVSAEGHVIDLNSEAARAGHFHLDPKTRGIGV
NRQGAFAEFVVAPAFNVIELPDDVPYEIGSILDPFGNAVHTAQQFDLLGEDVLVTGAGPI
GMMAAAVARHAGARTVVLTDINDFRLELAQKVAPGVRTVNTTKEDIHDVMKELGLKVGFD
VALEMSGSPIAFKQCVDTLIMGGGMALLGIPGKPMETDWGAIILKALTIKGVYGREMFTT
WRKMLGLLKAGLDLSPLITHRLGYADYREGFEAMKSGQSGKVVLDWDKAA