Protein Info for A4249_RS02455 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: acyl-CoA carboxylase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 76% identical to PCCB_RHOS4: Propionyl-CoA carboxylase beta chain (pccB) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K01966, propionyl-CoA carboxylase beta chain [EC: 6.4.1.3] (inferred from 93% identity to bsb:Bresu_1468)

MetaCyc: 76% identical to propionyl-CoA carboxylase beta subunit (Cereibacter sphaeroides)
Propionyl-CoA carboxylase. [EC: 6.4.1.3]

Predicted SEED Role

"Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 6.4.1.3)

MetaCyc Pathways

propanoyl CoA degradation I (3/3 steps found)
superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
2-oxobutanoate degradation I (3/4 steps found)
anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
3-hydroxypropanoate cycle (8/13 steps found)
methylaspartate cycle (12/19 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
superpathway of L-methionine salvage and degradation (9/16 steps found)
superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
mycolate biosynthesis (26/205 steps found)
superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.3

Use Curated BLAST to search for 6.4.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168N9U9 at UniProt or InterPro

Protein Sequence (511 amino acids)

>A4249_RS02455 acyl-CoA carboxylase subunit beta (Brevundimonas sp. GW460-12-10-14-LB2)
MTQAILEELERRRAAAKLGGGEKRIAAQHAKGKLTARERIDVLLDEGSFEETDMFVEHRS
HDFGMQDQRIPGDGVVTGRGTIGGRLVYVFSKDFTVFGGSLSGAHAAKIVKLQKMALTTG
APIIGLFDAGGARIQEGVESLAGYAEIFLQNTLASGVIPQISVIMGPCAGGDVYSPAITD
FIFMVKDTSYMYVTGPDVVKTVTNEVVSHEDLGGARVHAGKSGVADGAFENDLEALSEVR
RLIGFLPLSNREKPPVRESYDEPERDEPSLDTLIPANPNQPYDMKELILKVVDEADFFEI
GADFARNIICGFGRLDGQTVGIVANQPQVLAGVLDIDSSRKAARFVRFCDAFDIPLVTLV
DVPGFMPGTKQEYGALIKHGAKLLFAYAEATVPKLTLITRKAYGGAYDVMSSKHLRGDVN
YAWPTAEIAVMGAKGAVEIIFRKEAGDPEALAAREAEYKDRFANPFVAAGLGYIDDVIMP
HGTRRRLVKALRTLKNKVQENPWKKHDNIPL