Protein Info for A4249_RS02440 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: pyruvate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01064: pyruvate kinase
  • amino acids 5 to 467 (463 residues), 457.2 bits, E=2.7e-141
• PF00224: PK
  • amino acids 5 to 322 (318 residues), 335.7 bits, E=2.9e-104
• PF02887: PK_C
  • amino acids 355 to 467 (113 residues), 89.7 bits, E=1.4e-29

Best Hits

Swiss-Prot: 51% identical to KPYK_METEA: Pyruvate kinase (pyk) from Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)

KEGG orthology group: K00873, pyruvate kinase [EC: 2.7.1.40] (inferred from 86% identity to bsb:Bresu_3036)

Predicted SEED Role

"Pyruvate kinase (EC 2.7.1.40)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.1.40)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• Entner-Doudoroff pathway I (9/9 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• anaerobic energy metabolism (invertebrates, cytosol) (7/7 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• homolactic fermentation (10/12 steps found)
• Bifidobacterium shunt (12/15 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• Rubisco shunt (8/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• glycolysis VI (from fructose) (8/11 steps found)
• glycolysis IV (7/10 steps found)
• superpathway of glucose and xylose degradation (12/17 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
• heterolactic fermentation (12/18 steps found)
• glycolysis V (Pyrococcus) (6/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• mixed acid fermentation (10/16 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (4/9 steps found)
• glycerol degradation to butanol (9/16 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (11/19 steps found)
• superpathway of hexitol degradation (bacteria) (10/18 steps found)
• superpathway of N-acetylneuraminate degradation (11/22 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Glycolysis / Gluconeogenesis
• Purine metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.40

Sequence Analysis Tools

See A0A168N9T0 at UniProt or InterPro

Protein Sequence (481 amino acids)

>A4249_RS02440 pyruvate kinase (Brevundimonas sp. GW460-12-10-14-LB2)
MNRARRARIVATLGPASRAPSTVKALAQAGVDVFRLNFSHGSHEDHAAALKAVRGAEMAL
QRPLGVLADLQGPKLRLGRFKDVEIAVKPGHTMRFDLDPTPGDETRVQMPHPEIFKALRK
GMLLLLDDGRVRLRVGERTDEWADVTVESGSKLSDRKGVAVPEAVIPVSALTPKDREDLA
FALRMGVDWVALSFVQKPEDMAELKRIVNGQAACLAKIEKPAALADLEAILDYCDGVMVA
RGDLGVELDPEDVPVAQKQIVRAARNRGVPVIVATQMLESMTSAPAPTRAEATDVANAVY
EGADALMLSAETASGDYPLESVGIMSRIIERVERDPMWPSLMGAEHAGMDEHDVDALVGA
ARMAANAPSTGCLVVFTTLGGTARRMARERPLKPILVLTPNPNTARRLALVWGLEPRLGE
QPDSLEAVTDDAVNSAVKYGLAEPGQRILILAGTPFGAPGAANLLRLAHAPAATLKGRKK
S