Protein Info for A4249_RS02270 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 996 TIGR00392: isoleucine--tRNA ligase" amino acids 27 to 890 (864 residues), 796 bits, see alignment E=2.4e-243 PF00133: tRNA-synt_1" amino acids 47 to 696 (650 residues), 427.9 bits, see alignment E=5.6e-132 PF09334: tRNA-synt_1g" amino acids 70 to 216 (147 residues), 35.7 bits, see alignment E=6.7e-13 PF08264: Anticodon_1" amino acids 741 to 893 (153 residues), 86.2 bits, see alignment E=3.2e-28

Best Hits

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 85% identity to bsb:Bresu_2239)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168N937 at UniProt or InterPro

Protein Sequence (996 amino acids)

>A4249_RS02270 isoleucine--tRNA ligase (Brevundimonas sp. GW460-12-10-14-LB2)
MADAPENTSESTARDYRDTVFLPETPFPMRGGLPQKEPVILEQWGDLYGTLRAERQQQNA
PLYVLHDGPPYANGDIHIGHALNKTLKDFVVRSRFLLGYDVDYVPGWDCHGLPIEWKIEE
QFRAKGRRKDEVSKDEFRRACREYAGKYIDLQKDQFHRLGIVGDFANRYATMDFTSEAAI
VAEFHKFKNSGQLYRGSKPVMWSPVERTALADAEIEYHDHVSPTIWVKFPVTAMSDDHPD
DLLAFRSSTPSIVIWTTTPWTIPANRAISYGPEIAYGLYEVTAMEEGLEFAPWAKPGDRL
ILADKLAEEVFKAAKIAAWTRVYDINPSGLETAHPLAALDSGYGFSVPLLAGDHVTDDAG
TGFVHTAPGHGADDFEVWKAHGHHEVPDTVDPDGAYYPGVPLFAGLKVLETEGKKTGKFG
PANGAVMEKLIEAGNLLARGRLEHSYPHSWRSKAPIIFRNTPQWFIRMDQPLKSGDTLRE
TALNAIDATAFHPVAGKNRIRSMVEGRPDWLVSRQRAWGTPLAMFIDKQTGQPLHDPEVD
ARIVKAVAEHGADIWFTAPDSDFLGAHDPARYEKVEDILDVWFDSGSTHAFTLDPRLPEN
GYTGDRPAHWPADLYLEGSDQHRGWFQSSLLEGCGTRGRAPFKAVLTHGFTLDEKGEKMS
KSKGNTTDPLTIIKESGADILRLWVALVDYSDDQRIGKQILQTTVDAYRKLRNTVRYLLG
ALADFDEAERLPLDQMPPLERFILHRLHELDAQVRAAYGEYRFQDVVRPVLEFCAGDLSA
LYFDIRKDALYCDRPDSLRRRAARTVMHEVFMRLTAWLAPLTPFTMEEAWTTRFPDAGSN
CARVIPDTPAEWENETAYTKLKNLLPIREVVLGSLELARRDKMIGSSLDADVLIVLDDPA
LIQLVQDVAGSENPDQFMADFCIASVARLTRDDPQGPEVPFRLPEYAGIGALVRRSDNPK
CARSWRRVPDVGSDPAYPDLSARDADAVRYWEQTHA