Protein Info for A4249_RS01510 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: phospho-N-acetylmuramoyl-pentapeptide- transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 369 transmembrane" amino acids 27 to 49 (23 residues), see Phobius details amino acids 74 to 95 (22 residues), see Phobius details amino acids 101 to 118 (18 residues), see Phobius details amino acids 138 to 158 (21 residues), see Phobius details amino acids 177 to 197 (21 residues), see Phobius details amino acids 209 to 229 (21 residues), see Phobius details amino acids 248 to 265 (18 residues), see Phobius details amino acids 272 to 291 (20 residues), see Phobius details amino acids 297 to 320 (24 residues), see Phobius details amino acids 347 to 366 (20 residues), see Phobius details TIGR00445: phospho-N-acetylmuramoyl-pentapeptide-transferase" amino acids 41 to 369 (329 residues), 402.2 bits, see alignment E=1e-124 PF00953: Glycos_transf_4" amino acids 103 to 293 (191 residues), 121 bits, see alignment E=2.5e-39

Best Hits

Swiss-Prot: 69% identical to MRAY_CAUVN: Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K01000, phospho-N-acetylmuramoyl-pentapeptide-transferase [EC: 2.7.8.13] (inferred from 86% identity to bsb:Bresu_2779)

MetaCyc: 52% identical to phospho-N-acetylmuramoyl-pentapeptide-transferase (Escherichia coli K-12 substr. MG1655)
Phospho-N-acetylmuramoyl-pentapeptide-transferase. [EC: 2.7.8.13]

Predicted SEED Role

"Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)" in subsystem Peptidoglycan Biosynthesis (EC 2.7.8.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168N4E7 at UniProt or InterPro

Protein Sequence (369 amino acids)

>A4249_RS01510 phospho-N-acetylmuramoyl-pentapeptide- transferase (Brevundimonas sp. GW460-12-10-14-LB2)
MFYLLYLYYADVAHQYPLLNLVQYQTVRVALAMATAMIVAVAMGSRFINWIRAKQGRGQP
IRDDGPVSHLSKVGTPTMGGLMILAGIGVAVLLWGDLTNPYIWIVSFVTAAFGVLGFIDD
YAKVTKQTSAGLTSKQKLLAQTVVAVVAGVLTVLWMTVSPTSPGLETSIAFPFFKAVLLN
IGWFYVAFAAFTIVGFSNAVNLTDGLDGLATVPVMMAAGAFGVISYLAGNFVFAQYLQVH
HVPGAGELAIFCAAMIGGGAGFLWYNAPPAKIFMGDTGSLALGGALGAIAVTTKHELVLG
IVGGLFVMEAASVMIQVGYYKLTKKRIFLMAPVHHHFEKMGWPESTVVIRFWIIAGALAL
LGLSTLKLR