Protein Info for A4249_RS01495 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: penicillin-binding protein 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 transmembrane" amino acids 60 to 80 (21 residues), see Phobius details PF03717: PBP_dimer" amino acids 101 to 205 (105 residues), 42.4 bits, see alignment E=1.1e-14 PF00905: Transpeptidase" amino acids 264 to 559 (296 residues), 180.5 bits, see alignment E=4.5e-57

Best Hits

Swiss-Prot: 51% identical to FTSI_CAUVN: Probable peptidoglycan D,D-transpeptidase FtsI (ftsI) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K03587, cell division protein FtsI (penicillin-binding protein 3) [EC: 2.4.1.129] (inferred from 66% identity to bsb:Bresu_2782)

Predicted SEED Role

"Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)" in subsystem Bacterial Cell Division or Bacterial Cytoskeleton or Flagellum in Campylobacter or Peptidoglycan Biosynthesis (EC 2.4.1.129)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129

Use Curated BLAST to search for 2.4.1.129

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168N498 at UniProt or InterPro

Protein Sequence (594 amino acids)

>A4249_RS01495 penicillin-binding protein 2 (Brevundimonas sp. GW460-12-10-14-LB2)
MSVQDHRYHRPAPGADFQERLQGRLSPFWRWLTELVWRLEHGFERARADARPEEDTRVRI
FLILIVFSCVFGGLAIGASYKALFAPANGLGRGVNPNALVRGDLTDRNGELLATNIVHYG
LYIDPAEIWDRDLAYRQIRRALPRISAERLKKVLGGDRRLIALTGLTPQEKAAVHDLALG
GVTFEPEDRRAYPLGTSAVHLIGDADTGGQGVSGAELAFNDEIRAAGQRGEAFPLSIDLR
VQGVLENELAAAAIKNQAKGAVGIVTDVQTGEVLGMASWPTFNSADRGAAPDGATLNRAV
SGHYEMGSVFKTFTVAAGLDTGRADMNTLFDASQAFQIGDRKIKDFHAQNRVMTLEEVYL
HSSNIGTSQLAVQMGPNTMRDYFRRLGLLDAAKIELKESAKPVVPRRWDNSTLASLSFGY
GIMITPAQMVQAMGALTNGGRMIPLSLRKGGARNVQPQQVVTEETSRAILDLLRRNVVKG
SGGFADAPGLRVGGKTGSANKLVGGRYDPSHALGSFAAVFPVDGPLNGKRYAILIVMDEP
GTYPKTGAYVAAPAVHNIADRIAGFLGVERRDDRWRTASGEKIPQYQDVAGDGL