Protein Info for A4249_RS00980 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: pyruvate, phosphate dikinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 893 TIGR01828: pyruvate, phosphate dikinase" amino acids 4 to 885 (882 residues), 1424.7 bits, see alignment E=0 PF01326: PPDK_N" amino acids 22 to 365 (344 residues), 185.6 bits, see alignment E=2.2e-58 PF00391: PEP-utilizers" amino acids 436 to 517 (82 residues), 82.5 bits, see alignment E=2e-27 PF02896: PEP-utilizers_C" amino acids 532 to 883 (352 residues), 263.3 bits, see alignment E=4e-82

Best Hits

Swiss-Prot: 65% identical to PPDK_RHIME: Pyruvate, phosphate dikinase (ppdK) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01006, pyruvate,orthophosphate dikinase [EC: 2.7.9.1] (inferred from 73% identity to aex:Astex_3119)

Predicted SEED Role

"Pyruvate,phosphate dikinase (EC 2.7.9.1)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NVS4 at UniProt or InterPro

Protein Sequence (893 amino acids)

>A4249_RS00980 pyruvate, phosphate dikinase (Brevundimonas sp. GW460-12-10-14-LB2)
MMTQWVYGFGGGSADGDASMKNLLGGKGANLAEMSSLGLPVPPGFTVTTEVCTHYYANNE
TYPADLDAQVQAALAKVEGVVGKTFGDVENPLLVSVRSGARASMPGMMDTVLNLGLNDQT
VEGLAKLSGDRRFAFDSYRRFITMYSNVVLGLSHDDFEEVLDQHKDRLGVTVDTDLTAAD
WEKVVADYKAVVERELGHAFPQDPKDQLWGAVGAVFESWMNDRAKFYRRMHDIPESWGTA
VNVQSMVFGNMGETSATGVAFTRNPSTGEARLYGEFLINAQGEDVVAGIRTPQSLTKIGR
EEMGETAPSMEEAMPEVFAQFVDVVGKLESHYRDMQDIEFTVEQGRLWMLQTRNGKRTAK
SALKVAVDLAAEGVISQEEAISRVEPSALDQLLHPTLDPNAARTVVAAGLPASPGAATGK
IVFDADEAERMSGLGEAVILVREETSPEDIHGMHAARGIVTARGGMTSHAAVVARGMGRA
CVSGAGEIHIDEAKGVFTARGRTFKAGEVITIDGSKGEVLDGAVPMIEPELTGDFQTLMG
WADKVRRLKVRANAETPLDAKTARGFGAEGIGLCRTEHMFFDDTRIAAVREMILADDEKG
RRAALAKIAPFQKSDFVELFEIMAGLPVTVRLLDPPLHEFIPHTEEDIDALAASSGIDAE
KLKRRAKELHETNPMLGHRGCRLGVAYPEIYEMQVRAIIEAALEVKQKSGAAPLPEIMHP
LVALGLEMKYLRELTDRTAKAVFEETGQSIDYLVGTMIELPRAAIRAGDLAEYAQFFSFG
TNDLTQTTFGISRDDSGRFLQAYLDKGIFETDPFVRLDQDGVGGLIEIAAERGREVRPEV
KLGICGEHGGDPSSIAFCEQAGLDYVSCSPYRVPIARLAAAQAALNVEREKDR