Protein Info for A4249_RS00970 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: glycine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 721 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 3 to 719 (717 residues), 568.1 bits, see alignment E=2e-174 PF02092: tRNA_synt_2f" amino acids 4 to 578 (575 residues), 619.6 bits, see alignment E=5.9e-190 PF05746: DALR_1" amino acids 608 to 712 (105 residues), 37 bits, see alignment E=3.3e-13

Best Hits

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168N0V8 at UniProt or InterPro

Protein Sequence (721 amino acids)

>A4249_RS00970 glycine--tRNA ligase subunit beta (Brevundimonas sp. GW460-12-10-14-LB2)
MPQLLLEVFSEEIPARMQQGAARDLERMVSDRLKAAGLTWEALTTYAGPRRLTLVIDGLP
TATPDREEEVKGPRASAPEQALEGFLRKTGLTRDQLTERDGVLFAVLSSKGRATTDLVAE
TVDQVIRTFPWPKSMRWGSGTLRWVRPIKRILCLFDGQVVPFEIDGIQSDAITEGHRFLG
SGELLRVSDFVDYRTQLEKNFVLLDVADRKLRILEQAKAACAARGLALVDDDGLLDEVAG
LAEWPTPILGDMDPQFLALPPEVVRLSMKVHQKYFAVRDPSKDGLAPNFLVVANVEATDG
GKALAAGNSRVLSARLNDARFFWDEDQKVGFDAWNAKLSGVTFHAKLGTLAERVERIAAL
AREIAPLVGADPDEAETAARLSKADLLSGMVSEFPELQGIMGGYYARLAGHSDAIADAVR
DHYKPQGPADTVPTAPVTVAVALADKLDTLVGFFAIDEKPTGSKDPFALRRAALGVIRLL
IENGARLPVREIALFQAGLGFEQAAQRYAAQQIATAPKKVMFNGQEAELAGFIATPEDGL
KSVADFRPAELSAFFADRLTVLLRDQGQRHDLVAAVFALGDDDLVRIVRRVEALAAFLAT
DDGANLLAGYKRASNILRAEEKKGPIPTGMVQTGLPNQPEAETTLAFAVGAARTAVEAAL
ETEDFAAAMTALARLRAPVDRFFDDVLVNSDVPAERENRLKLLGQVRDVMGQVADFGQIA
G