Protein Info for A4249_RS00615 in Brevundimonas sp. GW460-12-10-14-LB2
Annotation: threonine ammonia-lyase, biosynthetic
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to ILVA_ECOLI: L-threonine dehydratase biosynthetic IlvA (ilvA) from Escherichia coli (strain K12)
KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 81% identity to bsb:Bresu_0963)MetaCyc: 56% identical to threonine deaminase (Escherichia coli K-12 substr. MG1655)
Threonine ammonia-lyase. [EC: 4.3.1.19]
Predicted SEED Role
"Threonine dehydratase biosynthetic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 4.3.1.19)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (12/13 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-methionine degradation II (2/3 steps found)
- L-threonine degradation V (1/2 steps found)
- L-threonine degradation I (3/6 steps found)
- superpathway of L-threonine metabolism (11/18 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.19
Use Curated BLAST to search for 4.3.1.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160I2C7 at UniProt or InterPro
Protein Sequence (504 amino acids)
>A4249_RS00615 threonine ammonia-lyase, biosynthetic (Brevundimonas sp. GW460-12-10-14-LB2) MQDTIRRILTARVYDVAEETPLDPLRRLSARLDRPVLLKREDLQPVFSFKIRGAYNKIAG LSEDELSRGVICASAGNHAQGVALAAARRDIPATIVMPTTTPGIKVAAVRALGGDVVLHG DSFDEAYAHARELEGRTGAAFIHPFDDPDVIAGQGTVGLEIARQHADPIEAIFVPIGGGG LAAGVAAIVKFLRPETRIIGVEPVDAPTMKSAIDAGQVVTLNEVGLFADGVAVRRAGTET FRLCKDLLDEIVLVDTDAICAAIKDIFDDSRAIAEPSGAVALAGLKAWAAAHPGRGSLIA VNSGANLNFDRLRHVAERAEIGEGAEALLAVAMKDDRDDYRRFLDDLDGRSVTEFNYRWS GDGQAQIFVGVALRNGTHERDDLIARLRTGGYAVEDMSDNETAKLHVRYMVGGRTVGLPH ERILRFQFPERPGAFLRFLNSLQPAWALTLFHYRNHGDDVGRVLAGISVPPEEQDHLTAA LANLGYPYVDETANPACRLFLDGA