Protein Info for b3699 in Escherichia coli BW25113

Name: gyrB
Annotation: DNA gyrase subunit B (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 TIGR01059: DNA gyrase, B subunit" amino acids 5 to 804 (800 residues), 1041.9 bits, see alignment E=0 PF02518: HATPase_c" amino acids 32 to 173 (142 residues), 64.4 bits, see alignment E=3.7e-21 PF00204: DNA_gyraseB" amino acids 221 to 390 (170 residues), 203.4 bits, see alignment E=5.7e-64 PF01751: Toprim" amino acids 419 to 531 (113 residues), 67.6 bits, see alignment E=2.8e-22 PF21249: GyrB_hook" amino acids 533 to 561 (29 residues), 67.8 bits, see alignment (E = 1.4e-22) PF18053: GyrB_insert" amino acids 564 to 729 (166 residues), 187 bits, see alignment E=7.8e-59 PF00986: DNA_gyraseB_C" amino acids 732 to 793 (62 residues), 107.8 bits, see alignment 7.2e-35

Best Hits

Swiss-Prot: 100% identical to GYRB_ECO57: DNA gyrase subunit B (gyrB) from Escherichia coli O157:H7

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 100% identity to ecp:ECP_3900)

MetaCyc: 100% identical to DNA gyrase subunit B (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AES6 at UniProt or InterPro

Protein Sequence (804 amino acids)

>b3699 DNA gyrase subunit B (NCBI) (Escherichia coli BW25113)
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEII
VTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
SVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSLETFTNVT
EFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNI
FYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKE
GYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEY
LLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDLAGLPGKLADCQERDPALSEL
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKG
KQEQYIKDDEAMDQYQISIALDGATLHTNASAPALAGEALEKLVSEYNATQKMINRMERR
YPKAMLKELIYQPTLTEADLSDEQTVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLF
EPIVRVRTHGVDTDYPLDHEFITGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFE
QALDWLVKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTL
MGDAVEPRRAFIEENALKAANIDI