Protein Info for b2715 in Escherichia coli BW25113

Name: ascF
Annotation: fused cellobiose/arbutin/salicin-specific PTS enzymes: IIB component/IC component (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 transmembrane" amino acids 105 to 131 (27 residues), see Phobius details amino acids 147 to 170 (24 residues), see Phobius details amino acids 178 to 197 (20 residues), see Phobius details amino acids 217 to 234 (18 residues), see Phobius details amino acids 254 to 282 (29 residues), see Phobius details amino acids 287 to 308 (22 residues), see Phobius details amino acids 331 to 349 (19 residues), see Phobius details amino acids 361 to 382 (22 residues), see Phobius details amino acids 389 to 412 (24 residues), see Phobius details amino acids 433 to 455 (23 residues), see Phobius details PF00367: PTS_EIIB" amino acids 11 to 43 (33 residues), 56.1 bits, see alignment (E = 2e-19) PF02378: PTS_EIIC" amino acids 113 to 396 (284 residues), 146.5 bits, see alignment E=1.1e-46 TIGR00852: PTS system, maltose and glucose-specific subfamily, IIC component" amino acids 154 to 441 (288 residues), 295 bits, see alignment E=5.9e-92

Best Hits

Swiss-Prot: 100% identical to PTIBC_ECOLI: PTS system arbutin-, cellobiose-, and salicin-specific EIIBC component (ascF) from Escherichia coli (strain K12)

KEGG orthology group: K02752, PTS system, arbutin-, cellobiose-, and salicin-specific IIB component [EC: 2.7.1.69] K02753, PTS system, arbutin-, cellobiose-, and salicin-specific IIC component (inferred from 100% identity to eco:b2715)

MetaCyc: 100% identical to beta-glucoside specific PTS enzyme IIBC component (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-153A [EC: 2.7.1.199]

Predicted SEED Role

"PTS system, arbutin-, cellobiose-, and salicin-specific IIBC component (EC 2.7.1.69)" in subsystem Beta-Glucoside Metabolism (EC 2.7.1.69)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.199 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P24241 at UniProt or InterPro

Protein Sequence (485 amino acids)

>b2715 fused cellobiose/arbutin/salicin-specific PTS enzymes: IIB component/IC component (NCBI) (Escherichia coli BW25113)
MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSD
NQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRIGAGILDALIGTMSPLIPA
IIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFFLPLMVAASAAIKFKTNMSLA
IAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDS
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGAL
WPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQT
ALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICGAVAGMAGLASHSMAAPGLFT
SVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAQARKYQSVQPTVAK
EVSLN