Protein Info for b0070 in Escherichia coli BW25113

Name: setA
Annotation: broad specificity sugar efflux system (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 392 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 48 to 70 (23 residues), see Phobius details amino acids 82 to 99 (18 residues), see Phobius details amino acids 105 to 127 (23 residues), see Phobius details amino acids 148 to 166 (19 residues), see Phobius details amino acids 168 to 168 (1 residues), see Phobius details amino acids 172 to 196 (25 residues), see Phobius details amino acids 217 to 236 (20 residues), see Phobius details amino acids 254 to 275 (22 residues), see Phobius details amino acids 283 to 301 (19 residues), see Phobius details amino acids 308 to 329 (22 residues), see Phobius details amino acids 341 to 363 (23 residues), see Phobius details amino acids 369 to 387 (19 residues), see Phobius details TIGR00899: sugar efflux transporter" amino acids 17 to 391 (375 residues), 602.4 bits, see alignment E=1.7e-185 PF07690: MFS_1" amino acids 19 to 301 (283 residues), 92.9 bits, see alignment E=9.6e-31 amino acids 220 to 385 (166 residues), 63.4 bits, see alignment E=9.4e-22

Best Hits

Swiss-Prot: 100% identical to SETA_ECOLI: Sugar efflux transporter A (setA) from Escherichia coli (strain K12)

KEGG orthology group: K03291, MFS transporter, SET family, sugar efflux transporter (inferred from 100% identity to eco:b0070)

MetaCyc: 100% identical to sugar exporter SetA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-82

Predicted SEED Role

"Putative transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P31675 at UniProt or InterPro

Protein Sequence (392 amino acids)

>b0070 broad specificity sugar efflux system (NCBI) (Escherichia coli BW25113)
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNAI
AGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRHYLTLITCGVLLASLANTA
MPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPLAFMLALNYGFTVMFSIAAGI
FTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPL
WISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIF
FNSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQG
AIAQSWGHFAVYWVIAVISVVALFLTAKVKDV