Protein Info for b4384 in Escherichia coli BW25113

Name: deoD
Annotation: purine nucleoside phosphorylase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00107: purine nucleoside phosphorylase
  • amino acids 5 to 234 (230 residues), 423.6 bits, E=9.5e-132
• PF01048: PNP_UDP_1
  • amino acids 16 to 223 (208 residues), 140.1 bits, E=4e-45

Best Hits

Swiss-Prot: 100% identical to DEOD_SHIBS: Purine nucleoside phosphorylase DeoD-type (deoD) from Shigella boydii serotype 4 (strain Sb227)

KEGG orthology group: K03784, purine-nucleoside phosphorylase [EC: 2.4.2.1] (inferred from 100% identity to eco:b4384)

MetaCyc: 100% identical to purine nucleoside phosphorylase (Escherichia coli K-12 substr. MG1655)
Purine-nucleoside phosphorylase. [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]

Predicted SEED Role

"Purine nucleoside phosphorylase (EC 2.4.2.1)" in subsystem Adenosyl nucleosidases or Deoxyribose and Deoxynucleoside Catabolism or Purine conversions (EC 2.4.2.1)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of purine deoxyribonucleosides degradation (7/7 steps found)
• purine ribonucleosides degradation (6/6 steps found)
• superpathway of pyrimidine deoxyribonucleosides degradation (6/6 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• adenine and adenosine salvage V (3/3 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• pyrimidine deoxyribonucleosides degradation (3/3 steps found)
• superpathway of guanine and guanosine salvage (3/3 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• ureide biosynthesis (5/7 steps found)
• nucleoside and nucleotide degradation (halobacteria) (3/6 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• arsenic detoxification (mammals) (8/17 steps found)
• salinosporamide A biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.1

Use Curated BLAST to search for 2.4.2.1 or 2.4.2.15

Sequence Analysis Tools

See P0ABP8 at UniProt or InterPro

Protein Sequence (239 amino acids)

>b4384 purine nucleoside phosphorylase (NCBI) (Escherichia coli BW25113)
MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISV
MGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIR
FKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVE
MEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE