Protein Info for b4376 in Escherichia coli BW25113

Name: osmY
Annotation: periplasmic protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 201 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF04972: BON" amino acids 55 to 122 (68 residues), 72.6 bits, see alignment E=1.3e-24 amino acids 135 to 200 (66 residues), 70.9 bits, see alignment E=4.4e-24

Best Hits

Swiss-Prot: 100% identical to OSMY_ECOLI: Osmotically-inducible protein Y (osmY) from Escherichia coli (strain K12)

KEGG orthology group: K04065, hyperosmotically inducible periplasmic protein (inferred from 100% identity to eco:b4376)

Predicted SEED Role

"Osmotically inducible protein OsmY"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AFH8 at UniProt or InterPro

Protein Sequence (201 amino acids)

>b4376 periplasmic protein (NCBI) (Escherichia coli BW25113)
MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVDSSMNKVGNFMDDSAIT
AKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEAVKVAKGVEGVTSVSDKLH
VRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVETTDGVVQLSGTVDSQAQSDR
AESIAKAVDGVKSVKNDLKTK