Protein Info for b4350 in Escherichia coli BW25113

Name: hsdR
Annotation: endonuclease R (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1170 PF04313: HSDR_N" amino acids 240 to 392 (153 residues), 29.4 bits, see alignment E=2.2e-10 PF04851: ResIII" amino acids 443 to 622 (180 residues), 126.3 bits, see alignment E=3.4e-40 PF18766: SWI2_SNF2" amino acids 449 to 648 (200 residues), 58.9 bits, see alignment E=1.8e-19 PF00270: DEAD" amino acids 454 to 626 (173 residues), 39 bits, see alignment E=2.1e-13 PF00271: Helicase_C" amino acids 715 to 830 (116 residues), 42.7 bits, see alignment E=1.8e-14 PF08463: EcoEI_R_C" amino acids 1005 to 1166 (162 residues), 106.4 bits, see alignment E=5.2e-34

Best Hits

Swiss-Prot: 100% identical to T1RK_ECOLI: Type I restriction enzyme EcoKI R protein (hsdR) from Escherichia coli (strain K12)

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 100% identity to eco:b4350)

MetaCyc: 100% identical to type I restriction enzyme EcoKI endonuclease subunit (Escherichia coli K-12 substr. MG1655)
Type I site-specific deoxyribonuclease. [EC: 3.1.21.3]

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P08956 at UniProt or InterPro

Protein Sequence (1170 amino acids)

>b4350 endonuclease R (RefSeq) (Escherichia coli BW25113)
MMNKSNFEFLKGVNDFTYAIACAAENNYPDDPNTTLIKMRMFGEATAKHLGLLLNIPPCE
NQHDLLRELGKIAFVDDNILSVFHKLRRIGNQAVHEYHNDLNDAQMCLRLGFRLAVWYYR
LVTKDYDFPVPVFVLPERGENLYHQEVLTLKQQLEQQVREKAQTQAEVEAQQQKLVALNG
YIAILEGKQQETEAQTQARLAALEAQLAEKNAELAKQTEQERKAYHKEITDQAIKRTLNL
SEEESRFLIDAQLRKAGWQADSKTLRFSKGARPEPGVNKAIAEWPTGKDETGNQGFADYV
LFVGLKPIAVVEAKRNNIDVPARLNESYRYSKCFDNGFLRETLLEHYSPDEVHEAVPEYE
TSWQDTSGKQRFKIPFCYSTNGREYRATMKTKSGIWYRDVRDTRNMSKALPEWHRPEELL
EMLGSEPQKQNQWFADNPGMSELGLRYYQEDAVRAVEKAIVKGQQEILLAMATGTGKTRT
AIAMMFRLIQSQRFKRILFLVDRRSLGEQALGAFEDTRINGDTFNSIFDIKGLTDKFPED
STKIHVATVQSLVKRTLQSDEPMPVARYDCIVVDEAHRGYILDKEQTEGELQFRSQLDYV
SAYRRILDHFDAVKIALTATPALHTVQIFGEPVYRYTYRTAVIDGFLIDQDPPIQIITRN
AQEGVYLSKGEQVERISPQGEVINDTLEDDQDFEVADFNRGLVIPAFNRAVCNELTNYLD
PTGSQKTLVFCVTNAHADMVVEELRAAFKKKYPQLEHDAIIKITGDADKDARKVQTMITR
FNKERLPNIVVTVDLLTTGVDIPSICNIVFLRKVRSRILYEQMKGRATRLCPEVNKTSFK
IFDCVDIYSTLESVDTMRPVVVRPKVELQTLVNEITDSETYKITEADGRSFAEHSHEQLV
AKLQRIIGLATFNRDRSETIDKQVRRLDELCQDAAGVNFNGFASRLREKGPHWSAEVFNK
LPGFIARLEKLKTDINNLNDAPIFLDIDDEVVSVKSLYGDYDTPQDFLEAFDSLVQRSPN
AQPALQAVINRPRDLTRKGLVELQEWFDRQHFEESSLRKAWKETRNEDIAARLIGHIRRA
AVGDALKPFEERVDHALTRIKGENDWSSEQLSWLDRLAQALKEKVVLDDDVFKTGNFHRR
GGKAMLQRTFDDNLDTLLGKFSDYIWDELA