Protein Info for b4180 in Escherichia coli BW25113

Name: rlmB
Annotation: 23S rRNA (Gm2251)-methyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 243 transmembrane" amino acids 217 to 237 (21 residues), see Phobius details TIGR00186: RNA methyltransferase, TrmH family, group 3" amino acids 1 to 242 (242 residues), 314.6 bits, see alignment E=2.1e-98 PF08032: SpoU_sub_bind" amino acids 5 to 78 (74 residues), 67.7 bits, see alignment E=9.3e-23 PF00588: SpoU_methylase" amino acids 96 to 236 (141 residues), 150.5 bits, see alignment E=3.4e-48

Best Hits

Swiss-Prot: 100% identical to RLMB_ECOL6: 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (rlmB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K03218, RNA methyltransferase, TrmH family [EC: 2.1.1.-] (inferred from 100% identity to eco:b4180)

MetaCyc: 100% identical to 23S rRNA 2'-O-ribose G2251 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11588 [EC: 2.1.1.185]

Predicted SEED Role

"23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-)" (EC 2.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.185

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P63177 at UniProt or InterPro

Protein Sequence (243 amino acids)

>b4180 23S rRNA (Gm2251)-methyltransferase (NCBI) (Escherichia coli BW25113)
MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALESQGVVIQLANRQYLDE
KSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDGVTDPHNLGACLRSADAAG
VHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLARTMRMLQEENIWIVGTAGEADH
TLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSVATGICLFEAVR
QRS