Protein Info for b4120 in Escherichia coli BW25113

Name: melB
Annotation: melibiose:sodium symporter (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 473 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 37 to 57 (21 residues), see Phobius details amino acids 78 to 100 (23 residues), see Phobius details amino acids 106 to 130 (25 residues), see Phobius details amino acids 150 to 171 (22 residues), see Phobius details amino acids 177 to 198 (22 residues), see Phobius details amino acids 236 to 259 (24 residues), see Phobius details amino acids 270 to 290 (21 residues), see Phobius details amino acids 297 to 319 (23 residues), see Phobius details amino acids 325 to 353 (29 residues), see Phobius details amino acids 374 to 397 (24 residues), see Phobius details amino acids 409 to 432 (24 residues), see Phobius details TIGR00792: sugar (Glycoside-Pentoside-Hexuronide) transporter" amino acids 8 to 450 (443 residues), 576.8 bits, see alignment E=1.2e-177 PF13347: MFS_2" amino acids 10 to 436 (427 residues), 271.3 bits, see alignment E=6e-85

Best Hits

Swiss-Prot: 100% identical to MELB_ECOLI: Melibiose carrier protein (melB) from Escherichia coli (strain K12)

KEGG orthology group: K11104, melibiose permease (inferred from 100% identity to eco:b4120)

MetaCyc: 100% identical to melibiose:H+/Na+/Li+ symporter (Escherichia coli K-12 substr. MG1655)
RXN-17755; TRANS-RXN-94; TRANS-RXN-94A; TRANS-RXN-94B; TRANS-RXN0-519; TRANS-RXN0-520

Predicted SEED Role

"Melibiose carrier protein, Na+/melibiose symporter" in subsystem Melibiose Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P02921 at UniProt or InterPro

Protein Sequence (473 amino acids)

>b4120 melibiose:sodium symporter (NCBI) (Escherichia coli BW25113)
MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDP
IMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQIVFVCVTYILWGMTY
TIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTLPFVNYVGGGDRGFGFQ
MFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQLSCLLGMA
LAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWA
GASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADIVDYGEYKL
HVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTL
FFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA