Protein Info for b4076 in Escherichia coli BW25113

Name: nrfG
Annotation: heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 198 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details

Best Hits

Swiss-Prot: 100% identical to NRFG_ECOLI: Formate-dependent nitrite reductase complex subunit NrfG (nrfG) from Escherichia coli (strain K12)

KEGG orthology group: K04018, formate-dependent nitrite reductase complex NrfG subunit (inferred from 100% identity to eco:b4076)

Predicted SEED Role

"Cytochrome c-type heme lyase subunit nrfG, nitrite reductase complex assembly" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P32712 at UniProt or InterPro

Protein Sequence (198 amino acids)

>b4076 heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG (NCBI) (Escherichia coli BW25113)
MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRDPLHQFASQQTPEAQLQ
ALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQ
ASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANYAQAIELWQKVMDLNSPRVNR
TQLVESINMAKLLQRRLD