Protein Info for b4055 in Escherichia coli BW25113
Name: aphA
Annotation: acid phosphatase/phosphotransferase, class B, non-specific (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to APHA_ECOLI: Class B acid phosphatase (aphA) from Escherichia coli (strain K12)
KEGG orthology group: K03788, acid phosphatase (class B) [EC: 3.1.3.2] (inferred from 100% identity to eco:b4055)MetaCyc: 100% identical to acid phosphatase / phosphotransferase (Escherichia coli K-12 substr. MG1655)
RXN0-3741 [EC: 3.1.3.89]; 3'-nucleotidase. [EC: 3.1.3.89, 3.1.3.6]; 5'-nucleotidase. [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5]; Deoxynucleotide 3'-phosphatase. [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34]; 3.1.3.34 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34]; 3.1.3.34 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34]; 3.1.3.34 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34]; 3.1.3.34 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34]; Phosphoserine phosphatase. [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34, 3.1.3.3]; Acid phosphatase. [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34, 3.1.3.3, 3.1.3.2]; 3.1.3.2 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34, 3.1.3.3, 3.1.3.2]; 3.1.3.- [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34, 3.1.3.3, 3.1.3.2]; RXN-14534 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34, 3.1.3.3, 3.1.3.2]
Predicted SEED Role
"NMN phosphatase (EC 3.1.3.5); Class B acid phosphatase precursor (EC 3.1.3.2)" (EC 3.1.3.2, EC 3.1.3.5)
MetaCyc Pathways
- purine nucleotides degradation II (aerobic) (11/11 steps found)
- superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
- adenosine nucleotides degradation II (5/5 steps found)
- purine nucleotides degradation I (plants) (10/12 steps found)
- adenosine nucleotides degradation I (7/8 steps found)
- guanosine nucleotides degradation II (4/4 steps found)
- guanosine nucleotides degradation III (4/4 steps found)
- inosine 5'-phosphate degradation (4/4 steps found)
- superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
- L-serine biosynthesis I (3/3 steps found)
- NAD salvage pathway III (to nicotinamide riboside) (3/3 steps found)
- superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
- phosphate acquisition (1/1 steps found)
- guanosine nucleotides degradation I (3/4 steps found)
- superpathway of purines degradation in plants (13/18 steps found)
- UTP and CTP dephosphorylation I (5/7 steps found)
- ureide biosynthesis (5/7 steps found)
- NAD salvage (plants) (5/11 steps found)
- tunicamycin biosynthesis (2/9 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Glycine, serine and threonine metabolism
- Nicotinate and nicotinamide metabolism
- Purine metabolism
- Pyrimidine metabolism
- Riboflavin metabolism
- gamma-Hexachlorocyclohexane degradation
Isozymes
Compare fitness of predicted isozymes for: 3.1.3.2, 3.1.3.3, 3.1.3.5
Use Curated BLAST to search for 3.1.3.2 or 3.1.3.3 or 3.1.3.34 or 3.1.3.5 or 3.1.3.6 or 3.1.3.89
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P0AE22 at UniProt or InterPro
Protein Sequence (237 amino acids)
>b4055 acid phosphatase/phosphotransferase, class B, non-specific (NCBI) (Escherichia coli BW25113) MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQAPIHWVSVAQIENSLAG RPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFWEKMNNGWDEFSIPKEVAR QLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPATNMNPVIFAGDKPGQNTKSQW LQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTYKPLPQAGAFGEEVIVNSEY