Protein Info for b4006 in Escherichia coli BW25113

Name: purH
Annotation: bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00355: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • amino acids 8 to 529 (522 residues), 908.3 bits, E=7.7e-278
• PF02142: MGS
  • amino acids 19 to 132 (114 residues), 100 bits, E=7e-33
• PF01808: AICARFT_IMPCHas
  • amino acids 137 to 459 (323 residues), 429.9 bits, E=7e-133

Best Hits

Swiss-Prot: 100% identical to PUR9_ECO55: Bifunctional purine biosynthesis protein PurH (purH) from Escherichia coli (strain 55989 / EAEC)

KEGG orthology group: K00602, phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase [EC: 2.1.2.3 3.5.4.10] (inferred from 100% identity to eco:b4006)

MetaCyc: 100% identical to bifunctional AICAR transformylase/IMP cyclohydrolase (Escherichia coli K-12 substr. MG1655)
IMP cyclohydrolase. [EC: 3.5.4.10]; Phosphoribosylaminoimidazolecarboxamide formyltransferase. [EC: 3.5.4.10, 2.1.2.3]

Predicted SEED Role

"IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.3, EC 3.5.4.10)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• One carbon pool by folate
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.3 or 3.5.4.10

Sequence Analysis Tools

See P15639 at UniProt or InterPro

Protein Sequence (529 amino acids)

>b4006 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) (Escherichia coli BW25113)
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGF
PEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLE
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEMDDNEGSLTLATRFDLAIKAF
EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKLDMRYGENSHQQAAFYI
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSI
LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ
NVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEELRVVTKRQPSEQELRDALFC
WKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH