Protein Info for b3829 in Escherichia coli BW25113

Name: metE
Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 5 to 312 (308 residues), 419.4 bits, E=1.1e-129
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 8 to 752 (745 residues), 1124.6 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 154 to 221 (68 residues), 25 bits, E=1.1e-09
  • amino acids 426 to 749 (324 residues), 551 bits, E=9.7e-170

Best Hits

Swiss-Prot: 100% identical to METE_ECOLI: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Escherichia coli (strain K12)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 100% identity to ecj:JW3805)

MetaCyc: 100% identical to cobalamin-independent homocysteine transmethylase (Escherichia coli K-12 substr. MG1655)
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase. [EC: 2.1.1.14]

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis I (5/5 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• S-adenosyl-L-methionine salvage II (2/3 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• L-methionine biosynthesis III (2/4 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Sequence Analysis Tools

See P25665 at UniProt or InterPro

Protein Sequence (753 amino acids)

>b3829 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (NCBI) (Escherichia coli BW25113)
MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLP
VGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN
TNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPVLLGPVTWLWLGKVKGEQFDR
LSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL
TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRAD
LTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLR
DALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRAR
FKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRPAPITVEWAKYA
QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE
PALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD
VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL
WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG